Four‐dimensional imaging and computer‐assisted track analysis of nuclear migration in root hairs of Arabidopsis thaliana
Four‐dimensional imaging and computer‐assisted track analysis of nuclear migration in root hairs of Arabidopsis thaliana
复制标题
拟南芥根毛核迁移的四维成像和计算机辅助轨迹分析
作者：
N. Bruaene;G. Joss;Olivier Thas;P. Oostveldt
核迁移是许多真核生物正常生长和发育所必需的基本机制。在这项研究中，拟南芥根毛被用作研究模型，以深入了解核迁移的动态。根毛是表皮细胞的长管状生长物，负责吸收水分和养分。在根毛发育过程中，核在凸起形成后迁移到毛发中。细胞核相对于尖端的位置在生长过程中起着至关重要的作用。然而，完全生长的根毛中的细胞核发生了什么仍不清楚。为了研究活根毛细胞的核动力学，使用了具有融合蛋白 Histone2B-YFP 和 NLS-GFP-GUS 的稳定转化植物。四维共焦激光扫描显微镜使得监测不同根毛中细胞核的精确位置成为可能。为了分析根毛中细胞核的顺序位置，开发了一种新的计算机辅助方法。经过轨迹分析后，可以从电影中提取许多参数，例如平均速度、幅度、方向因子和根毛的运动范围。我们的结果表明，细胞核在完全生长的根毛中并未到达最终位置，并且这种持续运动似乎在彼此靠近的根毛中更为相似。此外，通过这种方法可以定量地证明肌动蛋白的完整性对于核运动是必要的。
Nuclear migration is a fundamental mechanism necessary for the proper growth and development of many eukaryotic organisms. In this study root hairs of Arabidopsis thaliana were used as a research model to gain insight into the dynamics of nuclear migration. Root hairs are long tubular outgrowths of epidermal cells and are responsible for the uptake of water and nutrients. During the development of root hairs, the nucleus migrates into the hair after the bulge is formed. The position of the nucleus relative to the tip plays an essential role in the growth process. However, what is happening to the nucleus in full‐grown root hairs is still unclear. To study nuclear dynamics in living root hair cells, stably transformed plants with the fusion proteins Histone2B‐YFP and NLS‐GFP‐GUS were used. Four‐dimensional confocal laser scanning microscopy made it possible to monitor the exact position of the nucleus in different root hairs. To analyse the sequential positions of the nuclei in the root hairs, a new computer‐assisted method was developed. After track analysis a number of parameters could be extracted from the movies, such as the average speed, the amplitude, direction factor and the range of movement in the root hairs. Our results show that nuclei do not reach a final position in full‐grown root hairs and this sustained movement seems to be more similar in root hairs lying close to each other. Moreover, with this methodology it could be quantitatively demonstrated that the integrity of actin is necessary for nuclear movement.
使用激光显微手术切断姐妹着丝粒之间的着丝粒运动：证明着丝粒方向不稳定性和位置受张力调节。
影响因子：
4
作者：
Skibbens,RV;Rieder,CL;Salmon,ED
通讯作者：
Salmon,ED
影响因子：
7.4
作者：
Nebenführ, A;Gallagher, LA;Staehelin, LA
通讯作者：
Staehelin, LA