Oxidative stress in prostatic fluid of patients with chronic pelvic pain syndrome: correlation with gram positive bacterial growth and treatment response.
Oxidative stress in prostatic fluid of patients with chronic pelvic pain syndrome: correlation with gram positive bacterial growth and treatment response.
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DOI：
10.1002/j.1939-4640.2000.tb02135.x
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发表时间：
2000-09
影响因子：
--
通讯作者：
A. Shahed;D. Shoskes
中科院分区：
文献类型：
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作者：
A. Shahed;D. Shoskes
慢性盆腔疼痛综合征（CPPS）/慢性前列腺炎III类的病因仍然未知。尽管一部分男性对抗菌治疗有反应，但从前列腺分泌物（EPS）中分离出的革兰氏阳性菌通常被认为是细菌性的而不是致病性的。我们希望研究氧化应激作为组织损伤的标志物和CPPS男性EPS的反应，以确定革兰氏阳性菌感染是否与氧化应激增加有关。从100名男性CPPS共300 EPS标本进行显微镜检查，培养，生化和分子检测。通过8-异前列烷F2 α（IsoP）水平和总抗氧化能力作为Trolox当量来测量氧化损伤。从EPS的总RNA用于血红素加氧酶-1（HO-1）和颗粒酶B的基因表达。EPS中发现的唯一细菌为革兰氏阳性。在我们的分析中，这些男性被归类为慢性细菌性前列腺炎（II类）。IsoP水平（pg/mL）在II类前列腺炎（7315 +/- 1428）中最高，其次是非细菌性前列腺炎（IIIa类，2043 +/- 561），前列腺痛（IIIb类，319 +/- 81）和无症状对照（298 +/- 99）。IsoP水平在抗生素或抗氧化剂补充剂（Prosta-Q）成功治疗后显著下降。抗氧化能力分别在18名、16名和16名接受II类、IIIa类和IIIb类前列腺炎检测的男性中检测到11名、4名和1名。未观察到IsoP水平与EPS中白色血细胞数量之间的相关性。HO-1和颗粒酶B的表达在II型前列腺炎患者中最高，而在III型前列腺炎患者或无症状对照组中最低。根据氧化应激升高、对抗生素的临床反应以及治疗后氧化应激降低，我们得出结论，某些CPPS患者中的革兰氏阳性菌可能是病原体。据推测，氧化应激可能是一些患有CPPS的男性的一个关键途径，可以通过抗氧化治疗来靶向治疗。
The etiology of chronic pelvic pain syndrome (CPPS)/chronic prostatitis category III remains unknown. Whereas a subset of men respond to antimicrobial therapy, gram positive bacteria isolated from expressed prostatic secretions (EPS) are often considered to be commensal rather than pathogenic. We wished to study oxidative stress as a marker of tissue injury and response in EPS of men with CPPS to determine whether infection with gram positive bacteria is associated with increased oxidative stress. A total of 300 EPS specimens from 100 men with CPPS were collected for microscopy, culture, and biochemical and molecular assays. Oxidant injury was measured by 8-isoprostane F2alpha (IsoP) levels and total antioxidant capacity as Trolox equivalents. Total RNA from EPS was used for gene expression of heme oxygenase-1 (HO-1) and granzyme B. The only bacteria found in EPS were gram positive. For our analysis, these men were classified as having chronic bacterial prostatitis (category II). IsoP levels (pg/mL) were highest in men with category II prostatitis (7315 +/- 1428) followed by nonbacterial prostatitis (category IIIa, 2043 +/- 561), prostatodynia (category IIIb, 319 +/- 81), and asymptomatic controls (298 +/- 99). IsoP levels decreased significantly after successful treatment with antibiotics or an antioxidant supplement (Prosta-Q). Antioxidant capacity was detected in 11 out of 18, 4 out of 16, and 1 out of 16 men tested with category II, IIIa, and IIIb prostatitis, respectively. No correlation was observed between IsoP levels and the number of white blood cells in EPS. HO-1 and granzyme B expression was highest in men with category II prostatitis than in men with either category III prostatitis or asymptomatic controls. On the basis of elevated oxidative stress, clinical response to antibiotics, and post-treatment reduction in oxidative stress, we conclude that gram positive bacteria in some men with CPPS may be pathogens. It is speculated that oxidative stress may be a key pathway in some men with CPPS that can be targeted with antioxidant therapy.