Neutrophil CD11B expression and neutrophil activation in pre-eclampsia
Neutrophil CD11B expression and neutrophil activation in pre-eclampsia
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DOI：
10.1042/cs0920037
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发表时间：
1997-01-01
期刊：
影响因子：
6
通讯作者：
Michael, CA
中科院分区：
文献类型：
--
作者：
Barden, A;Graham, D;Michael, CA
1.采用全血流式细胞术检测22例子痫前期患者和22例年龄、孕周相匹配的正常对照中性粒细胞活化情况，以检测CD11b和CD18.2在基础状态和血小板活化因子刺激下的变化。子痫前期患者分娩前基础中性粒细胞CD11b的表达明显高于正常妊娠组，CD18的表达也无明显变化。分娩前，血小板活化因子刺激后，中性粒细胞CD11b的表达呈剂量依赖性增加，并维持组间差异，血小板活化因子刺激后，中性粒细胞CD18的表达也呈类似的剂量依赖性增加。在产后6周或6个月，无论是在血小板激活因子刺激之前还是之后，CD11b或CD18的表达在组间均无差异。子痫前期患者中性粒细胞CD11b与血尿酸呈正相关(r=0.44，P=0.04)，提示中性粒细胞活化程度与病情严重程度相关。子痫前期患者基础中性粒细胞CD11b表达的增加可能是体内中性粒细胞激活的一个指标，中性粒细胞释放自由基和蛋白酶可能有助于维持胎盘和全身循环中内皮和血管功能障碍的恶性循环。这种激活的原因尚不清楚，但可能与血小板激活、内皮素-L的产生增加或细胞因子的释放有关，需要进一步的研究来阐明中性粒细胞激活在子痫前期中的后果。
1. Neutrophil activation was examined in 22 women with pre-eclampsia and 22 age- and gestation-matched control subjects using whole-blood flow cytometry to assess basal and platelet-activating factor stimulated CD11b and CD18.2. Basal neutrophil CD11b expression was significantly increased in women with pre-eclampsia compared with normal pregnancy before delivery, A similar non-significant trend for CD18 was also observed.3. Before delivery, neutrophil CD11b expression increased in a dose-dependent fashion after platelet-activating factor stimulation, with the differences between the groups maintained, A similar dose-dependent increase in CD18 expression was observed after platelet-activating factor.4. There were no between-group differences in expression of either CD11b or CD18 at either 6 weeks or 6 months post partum, either before or after platelet-activating factor stimulation.5. Neutrophil CD11b was positively correlated with plasma uric acid (r = 0.44, P = 0.04) in women with pre-eclampsia, suggesting that the extent of neutrophil activation correlates with disease severity.6. An increase in basal neutrophil CD11b expression in women with pre-eclampsia is likely to be an index of neutrophil activation in vivo, Neutrophil release of free radicals and proteases may then help perpetuate a vicious cycle of endothelial and vascular dysfunction in the placental and systemic circulations. The cause of this activation is not known but could involve platelet activation, increased production of endothelin-l or release of cytokines, Further studies will be required to elucidate the consequences of neutrophil activation in pre-eclampsia.