A Multidrug-resistant Monophasic Salmonella Typhimurium Co-harboring mcr-1, fosA3, bla (CTX-M-14) in a Transferable IncHI2 Plasmid from a Healthy Catering Worker in China.
A Multidrug-resistant Monophasic Salmonella Typhimurium Co-harboring mcr-1, fosA3, bla (CTX-M-14) in a Transferable IncHI2 Plasmid from a Healthy Catering Worker in China.
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DOI：
10.2147/idr.s272272
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发表时间：
2020
影响因子：
3.9
通讯作者：
Jiao X
中科院分区：
文献类型：
--
作者：
Wang Z;Xu H;Tang Y;Li Q;Jiao X
多粘菌素目前被认为是根除多重耐药（MDR）革兰氏阴性菌的最后手段。同时，旧的抗菌药物磷霉素最近重新引入临床使用，用于治疗产超广谱β-内酰胺酶（ESBL）和耐碳青霉烯类肠杆菌科。本研究调查了来自食品餐饮处理者的多重耐药沙门氏菌4，[5]，12：i：-菌株，该菌株有可能作为传播MDR食源性病原体的载体。根据2003年世界卫生组织沙门氏菌检测方法标准方案，从某食品餐饮业从业人员粪便中分离到1株沙门氏菌4，[5]，12：i：-YZU 1189菌株。根据Kauffmann-White方案进行YZU 1189的血清分型。根据美国临床实验室标准化协会（CLSI）的指导，采用琼脂稀释法测定菌株的耐药表型。在供体菌株沙门氏菌4，[5]，12：i：-YZU 1189和受体菌株大肠杆菌C600之间进行质粒接合。通过全基因组序列分析确定mcr-1、blaCTX-M-14和fosA 3基因的遗传位置。沙门氏菌4，[5]，12：i：-YZU 1189是从健康饮食业工人中分离的产ESBL菌株。该菌株对氨基糖苷类、β-内酰胺类、多粘菌素类、磷霉素类、酚类、甲氧苄啶类、磺胺类、四环素类和氟喹诺酮类药物耐药。全基因组序列分析和质粒接合显示，该菌株具有携带mcr-1、blaCTX-M-14和fosA 3基因的可转移IncHI 2质粒。序列同源性分析表明，该质粒与国内已报道的mcr-1、blaCTX-M-14和fosA 3阳性质粒具有较高的同源性。本研究首次报道了我国分离的一株携带mcr-1、blaCTX-M-14和fosA 3的多重耐药沙门氏菌4，[5]，12：i：-。产ESBL沙门氏菌4，[5]，12：i：-分离株的可转移IncHI 2质粒pYZU 1189中出现mcr-1和fosA 3基因，显示对公共卫生存在潜在威胁。应高度关注多重耐药产ESBL沙门氏菌分离株从食品餐饮工作者向消费者的传播。
Polymyxins are currently regarded as a possible last-resort therapy to eradicate multidrug-resistant (MDR) gram-negative bacteria. Meanwhile, the old antimicrobial agent fosfomycin has recently been reintroduced into clinical use for the treatment of extended-spectrum β-lactamase (ESBL)-producing and carbapenem-resistant Enterobacteriaceae. This study investigated a multidrug-resistant Salmonella 4,[5],12:i:- strain from a food catering handler, which had the potential to act as a vehicle for transmitting MDR foodborne pathogens. A Salmonella 4,[5],12:i:- YZU1189 strain was isolated from the fecal sample of a food catering worker according to the standard protocol of the Salmonella detection method from World Health Organization in 2003. Serotyping of YZU1189 was performed according to the Kauffmann–White scheme. The antimicrobial resistance phenotype of the strain was determined by the agar dilution method according to the instruction from Clinical and Laboratory Standards Institute (CLSI). Plasmid conjugation was performed between the donor strain Salmonella 4,[5],12:i:- YZU1189 and the recipient strain Escherichia coli C600. The genetic locations of mcr-1, blaCTX-M-14 and fosA3 genes were determined by the whole genome sequence analysis. Salmonella 4,[5],12:i:- YZU1189 was an ESBL-producing stain isolated from a healthy catering worker. The strain displayed resistance to aminoglycosides, beta-lactams, polymyxins, fosfomycins, phenicols, trimethoprims, sulfonamides, tetracyclines and fluoroquinolones. Whole genome sequence analysis and plasmid conjugation revealed that the strain had a transferable IncHI2 plasmid carrying the mcr-1, blaCTX-M-14 and fosA3 genes. Sequence homology analysis showed that this plasmid possessed high sequence similarity to previously reported mcr-1, blaCTX-M-14 and fosA3 positive plasmids in China. This study reported a the multidrug-resistant Salmonella 4,[5],12:i:- isolate harboring mcr-1, blaCTX-M-14 and fosA3 from human for the first time in China. The occurrence of mcr-1 and fosA3 genes in the transferable IncHI2 plasmid pYZU1189 from the ESBL-producing Salmonella 4,[5],12:i:- isolate showed a potential threat to public health. Great concern should be taken for the spread of multidrug-resistant ESBL-producing Salmonella isolates from food catering workers to consumers.