Fine mapping of the genetic locus L1 conferring black pods using a chromosome segment substitution line population of soybean
Fine mapping of the genetic locus L1 conferring black pods using a chromosome segment substitution line population of soybean
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DOI：
10.1111/pbr.12272
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发表时间：
2015-08-01
期刊：
影响因子：
2
通讯作者：
Gai, Junyi
中科院分区：
文献类型：
--
作者：
He, Qingyuan;Yang, Hongyan;Gai, Junyi
植物器官的颜色在作物育种中是一个有用的性状。大豆的荚色主要包括黑色、棕色和棕褐色，这三种类型受两个经典的遗传位点L1和L2控制。大多数野生大豆有黑色豆荚，这反映了适应自然环境的可能作用。本研究建立了一个改良的染色体片段代换系（CSSL）群体SojaCSSLP 3，以鉴定L1基因。SSR标记Satt 313所代表的第19号染色体片段与L1位点连锁。使用来自SojaCSSLP 3的CSSL 124杂合植物的群体，用增加的SSR和InDel标记将该区域进一步划界三次。L1基因最终定位于SSR_19p09和Indel_19P7之间的184.43-kb区域。用qRT-PCR分析了该区域的13个推定基因。Glyma 19 g27460是具有MYB DNA结合结构域的SANT超家族的成员，其表达水平在黑色豆荚中显著上调，并且被认为是L1基因的最可能候选者。
The colour of plant organs is a useful trait in crop breeding. The pod colours of soybeans primarily include black, brown and tan types, which are controlled by two classical genetic loci, L1 and L2. Most wild soybeans have black pods, which reflect a possible role in adaptation to the natural environment. Here, an improved chromosome segment substitution line (CSSL) population SojaCSSLP3 was established to identify the L1 gene. The segment on the 19th chromosome represented by the SSR marker Satt313 was found to link with locus L1. The region was further delimited three times with increased SSR and InDel markers using a population derived from a heterozygous plant of CSSL124 from SojaCSSLP3. The L1 gene was finally located in a 184.43-kb region between SSR_19p09 and Indel_19P7. Thirteen putative genes in this region were analysed with qRT-PCR. The expression level of Glyma19g27460, which is a member of the SANT superfamily with a MYB DNA-binding domain, was significantly upregulated in black pods and was recognized to be the most likely candidate for the L1 gene.