Modulation of opioid system in C57 mice after repeated treatment with morphine and naloxone: biochemical and behavioral correlates.
Modulation of opioid system in C57 mice after repeated treatment with morphine and naloxone: biochemical and behavioral correlates.
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DOI：
10.1016/0024-3205(84)90638-6
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发表时间：
1984-04
期刊：
影响因子：
6.1
通讯作者：
N. Brunello;A. Volterra;A. D. Di Giulio;V. Cuomo;G. Racagni
中科院分区：
文献类型：
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作者：
N. Brunello;A. Volterra;A. D. Di Giulio;V. Cuomo;G. Racagni
C57 BL/6 J（C57）小鼠在吗啡给药后表现出特定的反应模式，例如对阿片类药物药理作用的耐受性的快速发展和单次注射药物后自发活动的增加。我们测定了吗啡和纳洛酮重复给药后甲硫氨酸脑啡肽含量和不同阿片受体的反应性。长期吗啡给药既不改变小鼠脑内甲硫氨酸脑啡肽水平，也不改变小鼠脑内阿片受体密度。与此相反，重复给予阿片受体拮抗剂纳洛酮，在纹状体和脑干中产生3 H-DHM和3 H-DADLE结合位点的数量显着增加，而不改变甲硫氨酸脑啡肽的浓度。行为学研究表明，吗啡诱导的自发活动增加在纳洛酮预处理的小鼠中增强，因此表明C57小鼠对吗啡的行为反应与纹状体中阿片受体数量增加之间可能存在相关性。
C57BL/6J (C57) mice display a particular pattern of responses following morphine administration, such as a rapid development of tolerance to the pharmacological action of the opiate and an increase in locomotor activity after a single injection of the drug. We have measured met-enkephalin content and the responsiveness of different opiate receptors after repeated administration of morphine and naloxone. Prolonged morphine administration changed neither met-enkephalin levels, nor the density of opiate receptors in mice brain. In contrast repeated administration of the opiate antagonist naloxone, produced a marked increase in the number of3H-DHM and3H-DADLE binding sites in striatum and brainstem without modifying met-enkephalin concentrations. Behavioral studies have indicated that the morphine-induced increase in locomotor activity is enhanced in naloxone pretreated mice, thus suggesting a possible correlation between the behavioral response to morphine in C57 mice and the higher number of opiate receptors in the striatum.