Expression and Purification of the Fusion Protein of TAT Protein Transduction Domain with a Zinc Finger that Specific to SV40 Promoter
Expression and Purification of the Fusion Protein of TAT Protein Transduction Domain with a Zinc Finger that Specific to SV40 Promoter
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SV40启动子特异性锌指TAT蛋白转导域融合蛋白的表达与纯化
DOI：
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发表时间：
2005
期刊：
影响因子：
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通讯作者：
H. Pei
中科院分区：
文献类型：
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作者：
H. Pei
蛋白转导结构域(PTD)，如TAT的PTD，可以将与之融合表达的多肽转导到细胞中。通过筛选锌指噬菌体展示文库，获得了针对SV40启动子9个碱基的锌指多肽。将该锌指序列插入原核表达载体pET-TAT-NLS，构建重组表达载体pET-TAT-NLS-clone3。融合蛋白以可溶形式表达，约占总蛋白的18%。通过亲和层析对融合蛋白进行纯化。
Protein transduction domains (PTDs), such as the PTD of TAT, may transduce peptides which fusion expressed with them into cells. A zinc finger peptide that specific to a 9 bp target on SV40 promoter was got by screening zinc finger phage display libraries. The sequence of this zinc finger was inserted into a prokaryotic expression vector named pET-TAT-NLS, generating the recombinant plasmid pET-TAT-NLS-clone3. The fusion protein was expressed in a soluable form, amount to about 18% of total proteins. The fusion protein was then purified by affinity chromatography.