Low RIN1 Expression in HCC Is Associated With Tumor Invasion and Unfavorable Prognosis
Low RIN1 Expression in HCC Is Associated With Tumor Invasion and Unfavorable Prognosis
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DOI：
10.1309/ajcpegwydd86wwjk
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发表时间：
2013-07-01
影响因子：
3.5
通讯作者：
Liu, Yongfeng
中科院分区：
文献类型：
--
作者：
He, Hui;Wu, Gang;Liu, Yongfeng
目的：探讨Ras和Rab相互作用因子1 （RIN1）的表达与肝细胞癌（HCC）预后的关系。方法：采用实时聚合酶链反应、Western blot和免疫组织化学检测配对HCC组织中RIN1的表达。采用转染法分析RIN1的功能。结果：我们发现，与相应的正常组织相比，HCC样本中RIN1蛋白的表达下调。RIN1表达下调也与侵袭和较差的总生存期（OS）有关。我们的多变量分析结果表明，RIN1状态是OS的重要预后因素。过表达RIN1也能抑制HepG2细胞的侵袭。RIN1与ABL2的表达可能呈正相关。结论：我们的研究结果表明，RIN1抑制HCC患者的肿瘤侵袭，当RIN1表达下调时，HCC的预后可能较差。
Objectives: To explore the association between the expression of Ras and Rab interactor 1 (RIN1) and the prognosis of hepatocellular carcinoma (HCC).Methods: RIN1 expression was detected in paired HCC tissues by real-time polymerase chain reaction, Western blot analysis, and immunohistochemistry. Transfection was applied to analyze the RIN1 function.Results: We found that expression of the RIN1 protein was downregulated in the HCC samples compared with the corresponding normal tissues. Downregulation of RIN1 expression was also associated with invasion and poor overall survival (OS). The results of our multivariate analysis indicated that the RIN1 status is a significant prognostic factor for OS. RIN1 overexpression also inhibited cell invasion in HepG2 cells. The expression between RIN1 and ABL2 may present a positive correlation.Conclusions: Our results demonstrate that RIN1 suppresses tumor invasion in HCC patients and that a poor prognosis for HCC is expected when RIN1 expression is downregulated.