Association of increased type I collagen expression and relative stromal overgrowth in mouse epididymis neonatally exposed to diethylstilbestrol
Association of increased type I collagen expression and relative stromal overgrowth in mouse epididymis neonatally exposed to diethylstilbestrol
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DOI：
10.1002/mrd.20347
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发表时间：
2005-11
影响因子：
2.5
通讯作者：
K. Yamazaki;H. Fukata;T. Adachi;H. Tainaka;Masao Kohda;M. Yamazaki;K. Kojima;K. Chiba;C. Mori;M. Komiyama
中科院分区：
文献类型：
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作者：
K. Yamazaki;H. Fukata;T. Adachi;H. Tainaka;Masao Kohda;M. Yamazaki;K. Kojima;K. Chiba;C. Mori;M. Komiyama
本研究的目的是调查的分子变化，在附睾形态学变化的基础上，新生儿暴露于强效合成雌激素，即己烯雌酚（DES）。新生雄性小鼠在前5天皮下注射DES或内源性雌激素，即17 β-雌二醇（E2）（5 µg/小鼠/天）。在2、4和8周龄时，解剖小鼠的附睾。在2周龄时，在DES给药小鼠的附睾中观察到特征性形态学异常，如相对基质过度生长，但在E2给药小鼠中未观察到。微阵列和真实的时间RT-PCR分析显示，与对照组相比，DES处理小鼠附睾中I型前胶原α 1（col 1a 1）和col 1a 2基因的表达水平在2周龄时显著上调。Western印迹分析显示，DES处理的小鼠附睾中的I型胶原蛋白表达水平在2周龄时升高。原位杂交分析显示，在对照组和DES-和E2-处理的小鼠中，在2周龄的附睾基质组织中检测到类似的col 1a 1 mRNA信号。附睾III型胶原蛋白（col 3a 1），这是在许多间质结缔组织以及I型胶原蛋白中发现的基因表达水平，在所有组的2周龄时没有变化。这些结果表明，I型胶原蛋白表达增加与DES处理小鼠附睾中的相对基质过度生长相关。摩尔Reprod. Dev.© 2005 Wiley利斯公司
The aim of this study was to investigate the molecular changes that underlie morphological changes in the epididymis following neonatal exposure to potent synthetic estrogen, namely diethylstilbestrol (DES). Newborn male mice were subcutaneously injected with DES or endogenous estrogen, namely 17 β‐estradiol (E2) (5 µg/mouse/day), for the first 5 days. At the age of 2, 4, and 8 weeks, epididymides of the mice were dissected. Characteristic morphological abnormality, such as relative stromal overgrowth, was observed at the age of 2 weeks in the epididymis of DES‐treated mice, but not in E2‐treated mice. Microarray and real‐time RT‐PCR analyses revealed that the expression levels of procollagen type I alpha 1 (col1a1) and col1a2 genes were markedly upregulated at the age of 2 weeks in the epididymis of DES‐treated mice in comparison with the control. Western blot analysis revealed that type I collagen protein expression level in epididymis of DES‐treated mice was elevated at the age of 2 weeks. In situ hybridization analysis revealed that the signals of col1a1 mRNA were detected similarly throughout the stromal tissue of epididymis at the age of 2 weeks in control and DES‐ and E2‐treated mice. The gene expression level of epididymal type III collagen (col3a1), which is found in many stromal connective tissues as well as type I collagen, did not change at the age of 2 weeks in all groups. These results suggest that the increased type I collagen expression is associated with the relative stromal overgrowth in the epididymis of DES‐treated mice. Mol. Reprod. Dev. © 2005 Wiley‐Liss, Inc.