PREVENTION OF CYTO-TOXICITY AND INHIBITION OF INTERCELLULAR COMMUNICATION BY ANTIOXIDANT CATECHINS ISOLATED FROM CHINESE GREEN TEA
PREVENTION OF CYTO-TOXICITY AND INHIBITION OF INTERCELLULAR COMMUNICATION BY ANTIOXIDANT CATECHINS ISOLATED FROM CHINESE GREEN TEA
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DOI：
10.1093/carcin/10.6.1003
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发表时间：
1989-06-01
期刊：
影响因子：
4.7
通讯作者：
KLAUNIG, JE
中科院分区：
文献类型：
--
作者：
RUCH, RJ;CHENG, SJ;KLAUNIG, JE
含有几种儿茶素的中国绿色茶的抗氧化剂级分（绿色茶抗氧化剂; GTA）先前已显示抑制12-0-十四烷酰基佛波醇-13-乙酸酯（TPA）诱导的小鼠皮肤中的肿瘤促进。在本研究中，GTA显示出对过氧化氢（H2 O2）和超氧化物自由基（O2-）具有抗氧化活性。GTA还可防止氧自由基和H2 O2诱导的细胞毒性，并抑制培养的B6 C3 F1小鼠肝细胞和人角质形成细胞（NHEK细胞）中的细胞间通讯。GTA（0.05-50 . mu.g.ml）以浓度依赖性方式防止百草枯（1-10 mM）和葡萄糖氧化酶（0.8-40 μ g/ml）对肝细胞的杀伤（通过乳酸脱氢酶释放测量）。GTA（50 μ g/ml）还防止百草枯（5 mM）、葡萄糖氧化酶（0.8 μ g/ml）和苯巴比妥（500 . mu.g.ml）对肝细胞间通讯的抑制。此外，GTA（50 μ g/ml）阻止TPA（100 ng/ml）对人角质形成细胞细胞间通讯的抑制。细胞毒性和细胞间通讯的抑制，两种可能的机制，肿瘤促进剂可能产生其促进作用，因此，防止GTA。这两种作用的抑制的前-osidant化合物可能建议的机制，GTA抑制肿瘤促进体内。
An antioxidant fraction of Chinese green tea (green tea antioxidant; GTA), containing several catechins, has been previously shown to inhibit 12-0-tetradecanoylphorbol-13-acetate (TPA)-induced tumor promotion in mouse skin. In the present study, GTA was shown to have antioxidative activity toward hydrogen peroxide (H202) and the superoxide radical (02-). GTA also prevented oxygen radical and H202-induced cytotoxicity and inhibition of intercellular communication in cultured B6C3F1 mouse hepatocytes and human keratinocytes (NHEK cells). GTA (0.05-50 .mu.g.ml) prevented the killing of hepatocytes (measured by lactate dehydrogenase release) by paraquat (1-10 mM) and glucose oxidase (0.8-40 .mu.g/ml) in a concentration-dependent fashion. GTA (50 .mu.g/ml) also prevented the inhibition of hepatocyte intercellular communication by paraquat (5 mM), glucose oxidase (0.8 .mu.g/ml), and phenobarbital (500 .mu.g.ml). In addition, GTA (50 .mu.g/ml) prevented the inhibition of intercellular communication in human keratinocytes by TPA (100 ng/ml). Cytotoxicity and inhibition of intercellular communication, two possible mechanisms by which tumor promoters may produce their promoting effects were therefore prevented by GTA. The inhibition of these two effects of pro-osidant compounds may suggest a mechanism by which GTA inhibits tumor promotion in vivo.