Drosophila melanogaster as a model for arbovirus infection of adult salivary glands.
Drosophila melanogaster as a model for arbovirus infection of adult salivary glands.
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DOI：
10.1016/j.virol.2020.01.010
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发表时间：
2020-01
期刊：
影响因子：
3.7
通讯作者：
W. Palmer;M. Dittmar;B. Gordesky-Gold;J. Hofmann;S. Cherry
中科院分区：
文献类型：
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作者：
W. Palmer;M. Dittmar;B. Gordesky-Gold;J. Hofmann;S. Cherry
虫媒病毒是对公共卫生的一种新的威胁。虫媒病毒通过节肢动物胃肠道传播到脊椎动物，最终感染节肢动物唾液腺。因此，唾液腺免疫影响虫媒病毒的传播;然而，这些免疫反应知之甚少。在这里，我们描述了实用ofDrosophila melanogaster作为唾液腺感染模型。首先，我们描述了使用唾液腺特异性驱动程序来启动RNA干扰或病毒复制子转基因。接下来，我们用虫媒病毒感染果蝇，发现了多种感染果蝇唾液腺的病毒，尽管效率很低。我们发现这种感染不受抗病毒RNA沉默的控制;因此，我们沉默了唾液腺中的一组免疫基因，但没有观察到感染的变化。这些数据表明果蝇可用于研究唾液腺感染，并且可能存在未探索的控制该组织感染的途径。
Arboviruses are an emerging threat to public health. Arbovirus transmission to vertebrates hinges on dissemination from the arthropod gastrointestinal tract, and ultimately infection of the arthropod salivary glands. Therefore, salivary gland immunity impacts arbovirus transmission; however, these immune responses are poorly understood. Here, we describe the utility ofDrosophila melanogasteras a salivary gland infection model. First, we describe the use of a salivary gland-specific driver to launch RNA interference or virus replicon transgenes. Next, we infect flies with an arbovirus panel and find multiple viruses that infectDrosophilasalivary glands, albeit inefficiently. We find that this infection is not controlled by antiviral RNA silencing; thus, we silence a panel of immune genes in the salivary glands, but do not observe changes in infection. These data suggest thatDrosophilamay be used to study salivary gland infection, and that there are likely unexplored pathways controlling infection of this tissue.