A small polyadenylated RNA (7 S RNA), containing a putative ribosome attachment site, maps near the origin of human mitochondrial DNA replication.
A small polyadenylated RNA (7 S RNA), containing a putative ribosome attachment site, maps near the origin of human mitochondrial DNA replication.
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DOI：
10.1016/0022-2836(81)90454-x
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发表时间：
1981-08
影响因子：
5.6
通讯作者：
D. Ojala;S. Crews;J. Montoya;R. Gelfand;G. Attardi
中科院分区：
文献类型：
--
作者：
D. Ojala;S. Crews;J. Montoya;R. Gelfand;G. Attardi
HeLa细胞线粒体DNA的轻（L）链序列编码小聚腺苷酸化RNA（7S RNA），通过作图和测序研究，已被精确定位在基因组的一个区域中，该区域在L链转录方向上紧接在复制起点之前，从该起点延伸219个核苷酸至20个核苷酸内。7 S RNA的5′端测序分析显示，存在两个主要物种，在这一端的大小相差一个核苷酸，可能还有三个次要物种。7 S RNA在其3′端附近似乎含有一个潜在的阅读框，可阅读23或24个氨基酸长的多肽。此外，在5′非编码区，已鉴定出11个核苷酸长的序列，其与12 S核糖体RNA的3′端附近的序列互补，并且可能代表核糖体附着位点。
The light (L) strand sequence of HeLa cell mitochondrial DNA which codes for a small polyadenylated RNA (7 S RNA) has been precisely localized, by mapping and sequencing studies, in a region of the genome which immediately precedes the origin of replication in the direction of L-strand transcription, extending from 219 nucleotides to within 20 nucleotides from this origin. A 5′-end sequencing analysis of 7 S RNA has revealed the presence of two major species differing in size by one nucleotide at this end, and possibly three minor species. The 7 S RNA appears to contain, near its 3′-end, a potential reading frame for a polypeptide 23 or 24 amino acids long. Furthermore, in the 5′ non-coding region, an 11-nucleotide long sequence has been identified which is complementary to a sequence near the 3′-end of the 12 S ribosomal RNA, and possibly represents a ribosome attachment site.