Fish model for assessing the in vivo estrogenic potency of the mycotoxin zearalenone and its metabolites
Fish model for assessing the in vivo estrogenic potency of the mycotoxin zearalenone and its metabolites
复制标题
DOI：
10.1016/s0048-9697(99)00275-2
复制
发表时间：
1999-09-15
影响因子：
9.8
通讯作者：
Goksoyr, A
中科院分区：
文献类型：
--
作者：
Arukwe, A;Grotmol, T;Goksoyr, A
玉米赤霉烯酮（ZEA）是一种由不同镰刀菌菌株产生的真菌毒素，其代谢产物（α-和β-玉米赤霉烯醇）在鱼体内的雌激素效能已被研究。雌激素性进行了评价，使用在体外竞争性受体结合试验和在体内诱导卵黄和zonagenesis，两个雌激素受体（ER）介导的反应，是鱼卵子发生的组成部分。α-玉米赤霉烯醇和ZEA在虹鳟鱼（Oncorhynchus mykiss）中的ER结合亲和力分别约为雌二醇的1/150和1/300。将幼年鲑鱼（萨尔莫salar）暴露于单次腹膜内注射ZEA、α-玉米赤霉烯醇和β-玉米赤霉烯醇（各自为1和10 mg/kg），并与注射雌二醇-17 β（E-2; 5 mg/kg）的鱼和对照进行比较。采用间接酶联免疫吸附试验（ELISA）与同源抗体，剂量依赖性的诱导卵黄蛋白原（Vtg）和蛋壳辐射蛋白（Zr-蛋白）的观察暴露后7天ZEA和α-玉米赤霉烯醇。β-玉米赤霉烯醇未升高血浆Vtg水平，但在最高剂量（10 mg/kg）下观察到血浆Zr-蛋白水平的非显著升高。通常，α-玉米赤霉烯酚和ZEA具有与E-2相比约50%的雌激素效力，并且它们的雌激素效力顺序（在体外受体竞争性结合和体内诱导Vtg和Zr蛋白水平方面）为：α-玉米赤霉烯酚> ZEA > β-玉米赤霉烯酚。我们的研究结果表明，Vtg和Zr-蛋白水平的血浆分析提供了一个合适的体内鱼模型，用于评估ZEA及其代谢产物的雌激素效力。(C)1999 Elsevier Science B. V.保留所有权利。
The in vivo estrogenic potency of zearalenone (ZEA), a mycotoxin produced by different strains of Fusarium fungi, and its metabolites (alpha- and beta-zearalenol), have been studied in fish. Estrogenicity was evaluated using an in vitro competitive receptor binding assay and in vivo induction of vitellogenesis and zonagenesis, two estrogen receptor (ER)-mediated responses that are integral aspects of fish oogenesis. The ER binding affinities of alpha-zearalenol and ZEA in rainbow trout (Oncorhynchus mykiss) were approximately 1/150 and 1/300 to that of estradiol, respectively. Juvenile salmon (Salmo salar) were exposed to a single intraperitoneal injection of ZEA, a-zearalenol and beta-zearalenol (each at 1 and 10 mg/kg) and compared to fish injected with estradiol-17 beta (E-2; 5 mg/kg) and controls. Using indirect enzyme-linked immunosorbent assay (ELISA) with homologous antibodies, a dose-dependent induction of vitellogenin (Vtg) and eggshell zona radiata proteins (Zr-proteins) were observed 7 days after exposure to ZEA and alpha-zearalenol. beta-Zearalenol did not elevate plasma Vtg levels, but a non-significant elevation of plasma Zr-proteins levels was observed at the highest dose (10 mg/kg). Generally, alpha-zearalenol and ZEA possess estrogenic potencies that are approximately 50% compared to that of E-2, and their order of estrogenic potency (in both in vitro receptor competitive binding and in vivo induction of Vtg and Zr-proteins levels) is: alpha-zearalenol > ZEA > beta-zearalenol. Our results show that blood plasma analysis of Vtg and Zr-proteins levels provides a suitable in vivo fish model for assessing the estrogenic potencies of ZEA and its metabolites. (C) 1999 Elsevier Science B.V. All rights reserved.