Regulations on growth and development in tomato cotyledon, flower and fruit via destruction of miR396 with short tandem target mimic
Regulations on growth and development in tomato cotyledon, flower and fruit via destruction of miR396 with short tandem target mimic
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通过短串联靶标模拟物破坏 miR396 对番茄子叶、花和果实生长发育的调控
DOI：
10.1016/j.plantsci.2016.02.012
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发表时间：
2016-06-01
期刊：
影响因子：
5.2
通讯作者：
Zhu, Benzhong
中科院分区：
文献类型：
--
作者：
Cao, Dongyan;Wang, Jiao;Zhu, Benzhong
尽管很多关于miR396功能的研究都集中在子叶和叶片的生长发育上，但对花和果实的研究很少，尤其是对肉质果实的研究，如番茄果实。本文结合生物信息学和转基因介导的方法，探讨了miR396在番茄植株生长发育过程中的作用。在番茄中，miR396有两个成熟型（miR396a和miR396b），且miR396a在子叶、花、萼片和果实中的表达量显著高于miR396b。一般来说，miR396通过生长调节因子（GRFs）调控植物的生长发育。在番茄中，对所有13个SlGRFs进行了系统发育、结构域和表达模式分析。为了进一步研究miR396的作用，我们在番茄中过表达STTM396a/396a-88，诱导miR396a和miR396b显著下调，靶基因GRFs普遍上调。结果，花、萼片和果实都明显变大了。最显著的是，转基因系3号和4号花后39 d的萼片长度分别增加了75%和81%，果实重分别增加了45%和39%。总之，这些结果揭示了miR396在调控番茄花和果实发育中的新作用，为提高番茄果实产量提供了新的潜在途径。2016爱思唯尔爱尔兰有限公司版权所有。
Despite many studies about functions of miR396 were concentrated on cotyledon and leaf growth and development, only few researches were focused on flower and fruit, especially for fleshy fruit, for example, tomato fruit. Here, the roles of miR396 throughout the growth and development of tomato plant were explored with combining bioinformatics and transgene-mediated methods. In tomato, miR396 had two mature types (miR396a and miR396b), and miR396a expressed significantly higher than miR396b in cotyledon, flower, sepal and fruit. Generally, plant growth and development were regulated by miR396 via growth-regulating factors (GRFs). In tomato, all 13 SlGRFs were analyzed comprehensively, including phylogeny, domain and expression patterns. To investigate the roles of miR396 further, STTM396a/396a-88 was over-expressed in tomato, which induced miR396a and miR396b both dramatical down-regulation, and the target GRFs general up-regulation. As a result, the flowers, sepals and fruits all obviously became bigger. Most significantly, the sepal length of transgenic lines #3 and #4 at 39 days post-anthesis was separately increased 75% and 81%, and the fruit weight was added 45% and 39%, respectively. Overall, these results revealed novel roles of miR396 in regulating flower and fruit development, and provided a new potential way for improving tomato fruit yield. (C) 2016 Elsevier Ireland Ltd. All rights reserved.