Direct and Rapid Detection by PCR ofErysipelothrix sp. DNAs Prepared from Bacterial Strains and Animal Tissues
Direct and Rapid Detection by PCR ofErysipelothrix sp. DNAs Prepared from Bacterial Strains and Animal Tissues
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通过 PCR 直接快速检测丹毒丝菌。
DOI：
10.1128/jcm.37.12.4093-4098.1999
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发表时间：
1999
影响因子：
9.4
通讯作者：
T. Ohyama
中科院分区：
文献类型：
--
作者：
K. Takeshi;S. Makino;T. Ikeda;N. Takada;Atsushi Nakashiro;K. Nakanishi;K. Oguma;Y. Katoh;H. Sunagawa;T. Ohyama
摘要 一种快速筛查丹毒丝菌的 PCR 方法。在屠宰场中进行的检测是在使用引物组 MO101-MO102 进行初始扩增后，使用四组物种特异性寡核苷酸引物进行的，该引物组扩增了所有四种丹毒丝菌物种的 16S rRNA 序列。确定编码rRNA基因簇的DNA序列，包括16S rRNA、23S rRNA和5S rRNA下游的非编码区，以便设计物种特异性PCR检测系统的引物。 [0123] 猪红斑丹毒丝菌血清变种2 (日本DNA数据库登录号AB019247)、扁桃体埃里毒丝菌血清变种7 (登录号AB019248)、猪红斑丹毒丝菌血清变种13 (登录号AB019249)和猪红斑丹毒丝菌血清变种的4.5-kb DNA序列之间的同源性红斑狼疮血清型 18（登录号 AB019250）范围为 96.0 至 98.4%。 PCR 扩增具有特异性，能够区分四种丹毒丝菌的 DNA。直接用病动物组织标本进行PCR检测的结果与培养试验的结果进行比较，PCR检测在5小时内完成。使用该物种特异性系统进行的测试基于 PCR 扩增，并使用编码 rRNA 基因簇的 DNA 序列进行测试，是一种准确、易于阅读的快速诊断丹毒丝菌的筛选方法。屠宰场感染。
ABSTRACT A PCR method for rapid screening of Erysipelothrix spp. in the slaughterhouse was carried out by using four species-specific sets of oligonucleotide primers after initial amplification with the primer set MO101-MO102, which amplifies the 16S rRNA sequences of all four Erysipelothrix species. The DNA sequences coding for the rRNA gene cluster, including 16S rRNA, 23S rRNA, and the noncoding region downstream of 5S rRNA, were determined in order to design primers for the species-specific PCR detection system. The homology among the 4.5-kb DNA sequences of the rRNA genes ofErysipelothrix rhusiopathiae serovar 2 (DNA Data Bank of Japan accession no. AB019247 ), E. tonsillarum serovar 7 (accession no. AB019248 ), E. rhusiopathiae serovar 13 (accession no. AB019249 ), and E. rhusiopathiae serovar 18 (accession no. AB019250 ) ranged from 96.0 to 98.4%. The PCR amplifications were specific and were able to distinguish the DNAs from each of the four Erysipelothrix species. The results of PCR tests performed directly with tissue specimens from diseased animals were compared with the results of cultivation tests, and the PCR tests were completed within 5 h. The test with this species-specific system based on PCR amplification with the DNA sequences coding for the rRNA gene cluster was an accurate, easy-to-read screening method for rapid diagnosis of Erysipelothrix sp. infection in the slaughterhouse.
影响因子：
56.9
作者：
SAIKI, RK;SCHARF, S;ARNHEIM, N
通讯作者：
ARNHEIM, N