In vivo observations of terminal Schwann cells at normal, denervated, and reinnervated mouse neuromuscular junctions
In vivo observations of terminal Schwann cells at normal, denervated, and reinnervated mouse neuromuscular junctions
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正常、去神经支配和再神经支配小鼠神经肌肉接头处末端许旺细胞的活体观察
DOI：
10.1002/(sici)1097-4695(19990205)38:2
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发表时间：
1999-02-05
期刊：
影响因子：
--
通讯作者：
Balice-Gordon, RJ
中科院分区：
文献类型：
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作者：
O'Malley, JP;Waran, MT;Balice-Gordon, RJ
我们发现了一种低分子质量的荧光染料，钙蓝AM酯(CB)，它在体内标记神经肌肉连接处的终末雪旺细胞，而不损害它们，这种染料被用来跟踪小鼠胸锁乳突肌神经肌肉连接处的终末雪旺细胞数天到数月。终末雪旺细胞体和突起在这些时间间隔内的空间分布是稳定的，大多数连接处的突起与运动神经末梢分支精确对准。神经切断3天后，用CB标记失神经肌肉中终末雪旺细胞所阐述的广泛突起。CB标记的终末雪旺细胞突起的数量和长度在去神经支配后3天至1个月之间减少，提示终末雪旺细胞突起仅在神经损伤后再神经支配的情况下暂时维持，而终末雪旺细胞突起在轴突萌出之前延长，这些过程持续到神经再支配完成。通过在再神经支配期间观察相同的连接两次，我们直接观察到轴突萌发使用现有的雪旺细胞突起和细胞体的链作为生长的底物。因此，CB可以用来监测终末雪旺细胞的动态行为，其与运动轴突及其终末的相互作用对于连接的稳态和修复是重要的。(C)1999年John Wiley&Sons，Inc.
We found a low-molecular-mass, fluorescent dye, Calcein blue am ester (CB), that labels terminal Schwann cells at neuromuscular junctions in vivo without damaging them, This dye was used to follow terminal Schwann cells at neuromuscular junctions in the mouse sternomastoid muscle over periods of days to months. Terminal Schwann cell bodies and processes were stable in their spatial distribution over these intervals, with processes that in most junctions were precisely aligned with motor nerve terminal branches. Three days after nerve cut, the extensive processes elaborated by terminal Schwann cells in denervated muscle were labeled by CB. The number and length of CB-labeled terminal Schwann cell processes decreased between 3 days and 1 month after denervation, suggesting that terminal Schwann cell processes are only transiently maintained in the absence of innervation, During reinnervation after nerve crush, however, terminal Schwann cell processes were extended in advance of axon sprouts, and these processes persisted until reinnervation was completed. By viewing the same junctions twice during reinnervation, we directly observed that axon sprouts used existing Schwann cell processes and chains of cell bodies as substrates for outgrowth. Thus, CB can be used to monitor the dynamic behavior of terminal Schwann cells, whose interactions with motor axons and their terminals are important for junction homeostasis and repair. (C) 1999 John Wiley & Sons, Inc.