In vitro measures of membrane changes reveal differences between red blood cells stored in saline-adenine-glucose-mannitol and AS-1 additive solutions: a paired study.
In vitro measures of membrane changes reveal differences between red blood cells stored in saline-adenine-glucose-mannitol and AS-1 additive solutions: a paired study.
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膜变化的体外测量揭示了储存在盐水-腺嘌呤-葡萄糖-甘露醇和 AS-1 添加剂溶液中的红细胞之间的差异：一项配对研究。
DOI：
10.1111/trf.12344
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发表时间：
2014
期刊：
影响因子：
2.9
通讯作者：
Norris,PhilipJ
中科院分区：
文献类型：
--
作者：
Sparrow,RosemaryL;Sran,Amrita;Healey,Geraldine;Veale,MargaretF;Norris,PhilipJ
背景盐水-腺嘌呤-葡萄糖-甘露醇 (SAGM) 和一种变体溶液 AS-1 已用于保存红细胞 (RBC) 30 多年。据称，这些红细胞成分具有相似的质量，但尚未报道配对研究。为了确定是否存在差异，对 SAGM RBC 和 AS-1 RBC 进行了配对研究，以识别膜变化，包括微粒 (MP) 定量和体外 RBC-内皮细胞 (EC) 相互作用。 研究设计和方法 合并和分割两个全血包，并制备 RBC（n = 6 对）。一组在 SAGM 中暂停，另一组在 AS-1 中暂停。样品是在 42 天的冷藏期间收集的。通过流式细胞术测量红细胞形状和大小以及血型糖蛋白 A (GPA)+ 和磷脂酰丝氨酸 (PS)+MP。通过体外流动灌注测定测定红细胞对 EC 的粘附。还测量了常规变量（pH、溶血）。结果与 SAGM RBC 相比，从第 14 天起，AS-1 RBC 的溶血量较低 (p < 0.04)、较低的 GPA+MP (p < 0.03) 和较低的 PS+MP (p < 0.03)。与 SAGM RBC 相比，AS-1 RBC 从第 28 天起具有更高的侧向散射 (p<0.02)。与 AS-1 RBC 相比，SAGM RBC 在储存第 28 天与 EC 的粘附性更强 (p = 0.04)，但在第 42 天发生逆转 (p = 0.02)。结论 SAGM RBC 在储存过程中损失更多膜。 SAGM RBC 在储存第 28 天时与 EC 的粘附性增强，而 AS-1 RBC 在第 42 天时粘附性更强。这些差异对输血后 SAGM RBC 和 AS-1 RBC 的功能和存活率的影响仍有待确定。
BackgroundSaline‐adenine‐glucose‐mannitol (SAGM) and a variant solution, AS‐1, have been used for more than 30 years to preserve red blood cells (RBCs). Reputedly these RBC components have similar quality, although no paired study has been reported. To determine whether differences exist, a paired study of SAGM RBCs and AS‐1 RBCs was conducted to identify membrane changes, including microparticle (MP) quantitation and in vitro RBC–endothelial cell (EC) interaction.Study Design and MethodsTwo whole blood packs were pooled and split and RBCs were prepared (n = 6 pairs). One pack was suspended in SAGM and one in AS‐1. Samples were collected during 42 days of refrigerated storage. RBC shape and size and glycophorin A (GPA)+and phosphatidylserine (PS)+MPs were measured by flow cytometry. RBC adhesion to ECs was determined by an in vitro flow perfusion assay. Routine variables (pH, hemolysis) were also measured.ResultsCompared to SAGM RBCs, AS‐1 RBCs had lower hemolysis (p < 0.04), lower GPA+MPs (p < 0.03), and lower PS+MPs (p < 0.03) from Day 14 onward. AS‐1 RBCs had higher (p < 0.02) side scatter from Day 28 onward compared to SAGM RBCs. SAGM RBCs were more adherent to ECs on Day 28 of storage compared to AS‐1 RBCs (p = 0.04), but reversed on Day 42 (p = 0.02).ConclusionSAGM RBCs lose more membrane during storage. SAGM RBCs had increased adherence to ECs on Day 28 of storage, while AS‐1 RBCs were more adherent on Day 42. The effect of these differences on the function and survival of SAGM RBCs and AS‐1 RBCs after transfusion remains to be determined.