SELF-ASSEMBLY OF A PLANT-CELL WALL INVITRO
SELF-ASSEMBLY OF A PLANT-CELL WALL INVITRO
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DOI：
10.1016/0022-2836(75)90170-9
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发表时间：
1975-01-01
影响因子：
5.6
通讯作者：
ROBERTS, K
中科院分区：
文献类型：
--
作者：
HILLS, GJ;PHILLIPS, JM;ROBERTS, K
开发了一种方法，可以将衣藻的细胞壁分解成其组分，然后在体外重新组装成与原始细胞壁在化学和结构上相同的产物。如氯化锂和高氯酸钠等促进剂将细胞壁分为两个部分，一个是不溶的无定形内壁层，它保持其完整性(按整个壁的重量计算为7.5%)，另一个是盐溶组分，包含负责细胞壁外层结晶层的均一糖蛋白。通过透析从分离的细胞壁中去除盐分，可以迅速恢复完整的重新组装的细胞壁。成功重组细胞壁的必要条件包括存在一个合适的表面，在该表面上存在一个递减的盐梯度，以及存在盐不溶和盐溶两种成分。盐溶糖蛋白本身可以在各种条件下自组装，形成具有完整细胞壁外层晶体结构特征的碎片。内壁层和盐溶糖蛋白都含有相似的氨基酸和糖(阿拉伯糖、半乳糖、甘露糖)，并且都含有羟脯氨酸。在细胞壁的体外重组的基础上，我们讨论了活细胞壁形态发生的某些方面。这篇通讯描述了第一个在体外重建植物细胞壁的案例，并表明非常大的细胞结构的组件能够通过简单的自组装过程来构建。
A method has been developed by which the cell wall ofChlamydomonas reinhardimay be dissociated into its components, and then reassembledin vitrointo a product that is chemically and structurally identical to the original cell wall. Chaotropic agents, such as lithium chloride and sodium perchlorate, separate the wall into two fractions, an insoluble amorphous inner wall layer, which retains its integrity (7.5% by weight of the complete wall) and a salt-soluble fraction containing the homogeneous glycoproteins responsible for the outer crystalline layers of the cell wall. Removal of the salt from dissociated walls by dialysis leads to the rapid recovery of complete reassembled cell walls. The conditions necessary for successful reconstitution of the cell wallin vitroinclude the presence of a suitable surface, across which a decreasing salt gradient exists, and the presence of both the salt-insoluble and the salt-soluble components. The salt-soluble glycoproteins alone can self-assemble under various conditions to form fragments that have the crystalline structure characteristic of the outer layers of the complete cell wall. Both the inner wall layer and the salt-soluble glyco-proteins have similar bulk amino acid and sugar (arabinose, galactose, mannose) compositions and both contain hydroxyproline. On the basis of thein vitroreconstitution of the cell wall we discuss certain aspects ofin vivocell wall morphogenesis. This communication describes the first case in which a plant cell wall has been reconstructedin vitro, and indicates that components of very large cellular structures are capable of being built by a simple self-assembly process.