Identification and characterization of transcription factor ZmEREB94 involved in starch synthesis in maize
Identification and characterization of transcription factor ZmEREB94 involved in starch synthesis in maize
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DOI：
10.1016/j.jplph.2017.04.016
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发表时间：
2017-09-01
影响因子：
4.3
通讯作者：
Huang, Yubi
中科院分区：
文献类型：
--
作者：
Li, Hui;Xiao, Qianlin;Huang, Yubi
玉米是一种重要的粮食作物和工业原料，淀粉含量高。然而，淀粉合成的机制尚未完全阐明，特别是关于淀粉合成基因的表达和调控。APETALA 2/乙烯应答因子（AP 2/ERF）家族通过调节基因表达在多种生物学过程中起着至关重要的作用。本研究通过共表达分析鉴定了ZmEREB 94基因。生物信息学分析证实ZmEREB 94属于AP 2/ERF家族。表达谱分析表明，该蛋白在玉米胚乳中强烈表达。ZmEREB 94-GFP融合蛋白定位于洋葱表皮细胞核中，ZmEREB 94具有较强的转录激活活性，表明该蛋白是一种转录因子。此外，酵母单杂交试验和玉米胚乳中的瞬时表达表明，ZmEREB 94可以直接结合ZmSSI启动子并间接调节ZmSh 2和ZmGBSSI表达。我们的研究结果表明，ZmEREB 94可能是玉米淀粉合成的关键调节因子。
Maize is an important food crop and industrial material owing to its high starch content. However, the mechanism of starch synthesis is not fully elucidated, especially with regard to the expression and regulation of starch synthetic genes. The APETALA2/Ethylene Responsive Factor (AP2/ERF) family plays a crucial role in various biological processes via regulating gene expression. In this study, the ZmEREB94 gene was identified through co-expression analysis. Bioinformatics analysis confirmed that ZmEREB94 belongs to the AP2/ERF family. Expression pattern analysis showed that this protein is strongly expressed in the maize endosperm. A ZmEREB94-GFP fusion protein was localized in the nuclei of onion epidermal cells, and ZmEREB94 showed strong transcriptional activation activity, which indicated that this protein is a transcription factor. In addition, yeast-one hybrid assays and transient expression in maize endosperm showed that ZmEREB94 could directly bind to the ZmSSI promoter and indirectly regulate ZmSh2 and ZmGBSSI expression. Our results revealed that ZmEREB94 might act as a key regulator of starch synthesis in maize.