Plasmon-Enhanced Characterization of Single Extracellular Vesicles.
Plasmon-Enhanced Characterization of Single Extracellular Vesicles.
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DOI：
10.1007/978-1-0716-3203-1_1
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发表时间：
2023
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细胞外囊泡（EV）代表从几乎所有种类的细胞脱落的膜结合囊泡的异质群体。尽管上级传统方法，但大多数新开发的EV传感平台仍然需要一定数量的EV，测量来自一组囊泡的批量信号。一种新的分析方法，使单一的EV分析可能是非常有价值的了解EV的亚型，异质性和生产动态在疾病的发展和进展。在这里，我们描述了一种新的纳米等离子体传感平台，用于灵敏的单EV分析。该系统名为nPLEX-FL（具有增强荧光检测功能的纳米等离子体EV分析），使用周期性金纳米孔结构放大EV的荧光信号，从而实现对单个EV的灵敏、多路分析。
Extracellular vesicles (EVs) represent heterogeneous populations of membrane-bound vesicles shed from almost all kinds of cells. Although superior to conventional methods, most newly developed EV sensing platforms still require a certain number of EVs, measuring bulk signals from a group of vesicles. A new analytical approach that enables single EV analysis could be extremely valuable for understanding EVs' subtypes, heterogeneity, and production dynamics during disease development and progression. Here, we describe a new nanoplasmonic sensing platform for sensitive single EV analysis. Termed nPLEX-FL (nano-plasmonic EV analysis with enhanced fluorescence detection), the system amplifies EVs’ fluorescence signals using periodic gold nanohole structures, enabling sensitive, multiplexed analysis of single EVs.