Optogenetic approaches to cell migration and beyond.
Optogenetic approaches to cell migration and beyond.
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DOI：
10.1016/j.ceb.2014.08.004
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发表时间：
2014-10
影响因子：
7.5
通讯作者：
Hahn KM
中科院分区：
文献类型：
--
作者：
Weitzman M;Hahn KM
光遗传学，使用遗传编码工具来控制蛋白质的光功能，可以在活细胞和动物体内产生局部信号变化。多年来，它一直专注于神经生物学的通道蛋白，但最近已经扩展到涵盖许多不同类型的蛋白质，使用广泛的不同蛋白质工程方法。这些方法主要针对参与运动、细胞骨架调节和基因表达的蛋白质。这篇综述提供了一个调查的非通道蛋白，已被工程化的光遗传学。现有的分子被用来说明许多富有想象力的新方法的优点和缺点，读者可以用来创建光控蛋白质。
Optogenetics, the use of genetically encoded tools to control protein function with light, can generate localized changes in signaling within living cells and animals. For years it has been focused on channel proteins for neurobiology, but has recently expanded to cover many different types of proteins, using a broad array of different protein engineering approaches. These methods have largely been directed at proteins involved in motility, cytoskeletal regulation and gene expression. This review provides a survey of non-channel proteins that have been engineered for optogenetics. Existing molecules are used to illustrate the advantages and disadvantages of the many imaginative new approaches that the reader can use to create light-controlled proteins.