DHA enhances the noradrenaline release by SH-SY5Y cells
DHA enhances the noradrenaline release by SH-SY5Y cells
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DOI：
10.1016/j.neuint.2009.09.006
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发表时间：
2010-01-01
影响因子：
4.2
通讯作者：
Sylvie, Vancassel
中科院分区：
文献类型：
--
作者：
Geraldine, Mathieu;Stephanie, Denis;Sylvie, Vancassel
多不饱和脂肪酸（PUFAs），特别是二十二碳六烯酸（DHA）和花生四烯酸（AA），是脑膜磷脂的主要成分。饮食诱导的脑DHA缺失导致啮齿动物行为和神经传递的改变。为了确定PUFA是否对神经递质释放机制起作用，我们测量了PUFA膜含量改变的SH-SY5Y神经母细胞瘤细胞和含有高DHA或AA培养液的细胞中[H-3]-去甲肾上腺素（NA）的释放。与70 μ M DHA孵育3 d的细胞膜中乙醇胺甘油磷脂中DHA含量增加7.6倍，而与AA孵育3 d的细胞膜中DHA含量减少40%。DHA的掺入促进了[H-3]-NA的基础释放（25%,p < 0.05），但对kcl诱导的[H-3]-NA释放没有作用。在囊泡动员过程中与DHA短暂孵育也强烈增加了[H-3]-NA的释放。AA没有效果。编码钙传感器synaptotagmin 1和两个SNARE复合体蛋白syntaxin1A和synaptobrevin1的基因不受PUFA掺入的影响，这是对特定mrna和蛋白质的检测表明的。因此，高膜DHA含量和培养基中的游离DHA都能促进SH-SY5Y细胞释放[H-3]-NA。这表明脑膜DHA影响胞外分泌，进而调节神经传递。2009爱思唯尔有限公司版权所有。
Polyunsaturated fatty acids (PUFAs), particularly docosahexaenoic acid (DHA) and arachidonic acid (AA), are the main components of the phospholipids, in cerebral membranes. A dietary-induced cerebral DHA deficit results in altered behaviour and neurotransmission in rodents. To determine whether PUFA were acting on the neurotransmitter release machinery, we measured the release of [H-3]-noradrenaline (NA) from SH-SY5Y neuroblastoma cells with modified PUFA membrane contents and from cells incubated with medium containing high DHA or AA. The membranes of cells incubated with 70 mu M DHA for 3 days had 7.6-times more DHA in their ethanolamine glycerophospholipids, while the membranes of cells incubated with AA had 40% less. Incorporation of DHA enhanced basal [H-3]-NA release (25%, p < 0.05), but not KCl-evoked [H-3]-NA release. Brief incubation with DHA during vesicle mobilization also strongly increased [H-3]-NA release. AA had no effect. The genes encoding for the calcium sensor synaptotagmin 1, and for the two SNARE complex proteins syntaxin1A and synaptobrevin1 were not affected by PUFA incorporation, as indicated by assays for specific mRNAs and proteins. Thus both a high membrane DHA content and free DHA in the medium enhance the release of [H-3]-NA from SH-SY5Y cells. This suggests that brain membrane DHA influences exocytosis, which then regulates neurotransmission. (C) 2009 Elsevier Ltd. All rights reserved.