A NEW HERBACEOUS HOST OF CITRUS LEAF BLOTCH VIRUS
A NEW HERBACEOUS HOST OF CITRUS LEAF BLOTCH VIRUS
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DOI：
10.4454/jpp.v91i2.983
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发表时间：
2009-07
影响因子：
2.2
通讯作者：
M. Guardo;O. Potere;M. Castellano;V. Savino;A. Caruso
中科院分区：
文献类型：
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作者：
M. Guardo;O. Potere;M. Castellano;V. Savino;A. Caruso
柑桔叶斑病毒（Citrus leaf blotch virus，CLBV）是柑桔病毒属Citrivirus的一个新属，它能引起嫁接在三叶砧木上的长见橘和Calamondin接穗的芽愈合障碍。利用已侵染的长见橘和香橼的叶提取物成功地将该病毒传至凹腔烟草，从而扩大了其草本寄主范围。感染是潜伏的，但通过使用病毒特异性引物的RTPCR的阳性反应和通过电子显微镜证实。CLBV对N. cavicola原则上应有助于实验室调查，因为它提供了一种新的病毒来源，可替代柑橘，而且比柑橘更易于管理。
Citrus leaf blotch virus (CLBV), the type species of the putative new genus Citrivirus, causes a bud union disorder of Nagami kumquat and Calamondin scions grafted on trifoliate rootstocks. This virus was successfully transmitted to Nicotiana cavicola using leaf extracts of infected Nagami kumquat and Etrog citron, thus widening its herbaceous host range. The infection was latent but confirmed by the positive response of RTPCR using virus-specific primers and by electron microscopy. The positive transmission of CLBV to N. cavicola should in principle facilitate laboratory investigations, as it provides a new source of virus alternative to and more manageable than citrus.