A direct method for e-cigarette aerosol sample collection.
A direct method for e-cigarette aerosol sample collection.
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DOI：
10.1016/j.envres.2016.05.008
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发表时间：
2016-08
影响因子：
8.3
通讯作者：
Rule A
中科院分区：
文献类型：
--
作者：
Olmedo P;Navas-Acien A;Hess C;Jarmul S;Rule A
电子烟的使用在世界各地的人口中越来越多。最近的证据表明，电子烟产生的气溶胶可能含有多种有毒物质。已发表的表征电子烟气溶胶中有毒物质的研究依赖于过滤器，撞击器或吸附管，这些方法需要在收集过程中稀释或提取溶液中的样品。我们开发了一种收集系统，可以直接浓缩电子烟气溶胶样品进行化学和毒理学分析。收集系统由几个切割的移液器吸头与短管连接组成。基于移液器吸头的收集系统可以连接到蠕动泵、真空泵或直接连接到电子烟用户，以使电子烟气溶胶流过系统。基于移液器吸头的系统冷凝由电子烟产生的气溶胶，并收集准备用于分析的液体样品，而不需要中间提取溶液。我们测试了来自马里兰州市售的5个不同品牌的20支电子烟。在150次抽吸之后，基于移液管吸头的收集系统冷凝在0.23 mL与0.53 mL之间的蒸发后电子烟液。所提出的方法具有高度适应性，可在现场工作和实验环境中使用，并允许从各种电子烟设备中收集气溶胶样品，产生可能被输送到肺部的确切物质的冷凝物。
E-cigarette use is increasing in populations around the world. Recent evidence has shown that the aerosol produced by e-cigarettes can contain a variety of toxicants. Published studies characterizing toxicants in e-cigarette aerosol have relied on filters, impingers or sorbent tubes, which are methods that require diluting or extracting the sample in a solution during collection. We have developed a collection system that directly condenses e-cigarette aerosol samples for chemical and toxicological analyses. The collection system consists of several cut pipette tips connected with short pieces of tubing. The pipette tip-based collection system can be connected to a peristaltic pump, a vacuum pump, or directly to an e-cigarette user for the e-cigarette aerosol to flow through the system. The pipette tip-based system condenses the aerosol produced by the e-cigarette and collects a liquid sample that is ready for analysis without the need of intermediate extraction solutions. We tested a total of 20 e-cigarettes from 5 different brands commercially available in Maryland. The pipette tip-based collection system condensed between 0.23 and 0.53 mL of post-vaped e-liquid after 150 puffs. The proposed method is highly adaptable, can be used during field work and in experimental settings, and allows collecting aerosol samples from a wide variety of e-cigarette devices, yielding a condensate of the likely exact substance that is being delivered to the lungs.