Effect of oxygen concentrations and branched-chain amino acids on the growth and development of sub-seafloor fungus, Schizophyllum commune 20R-7-F01
Effect of oxygen concentrations and branched-chain amino acids on the growth and development of sub-seafloor fungus, Schizophyllum commune 20R-7-F01
复制标题
氧浓度和支链氨基酸对海底真菌裂褶菌 20R-7-F01 生长发育的影响
DOI：
10.1111/1462-2920.15738
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发表时间：
2021
影响因子：
5.1
通讯作者：
Liu Chang-Hong
中科院分区：
文献类型：
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作者：
Zain Ul Arifeen Muhammad;Ma Zhi-Jun;Wu Si;Liu Jun-Zhong;Xue Ya-Rong;Liu Chang-Hong
据报道，真菌是缺氧海底沉积物中占主导地位的真核生物群，但真菌如何在缺氧海底沉积环境中生存却很少了解。我们之前的研究表明，从海底以下约2公里的沉积物中分离出的真菌Schizophyllum commune 20 R-7-F01可以在完全没有氧气的情况下生长和产生原基，并提高支链氨基酸（BCAAs）的产量，但原基在没有氧气的情况下不能发育成子实体。在这里，我们介绍了氧气和支链氨基酸对该菌株子实体发育的单独和协同作用。结果表明，该菌产生原基所需的最低氧浓度为0.5%pO2，原基转化为成熟子实体所需的最低氧浓度为1%pO2。而在培养基中添加20 mM的BCAAs，原基在较低的氧浓度（pO_2为0.5%）下仍能发育成子实体，其中基因fst_4和c_2h_2起着补偿氧缺乏的重要作用。此外，在缺氧条件下，真菌表现出线粒体数量的增加和自吞噬作用的启动。这些结果表明，子实体的形成S。群落可能具有多种机制，包括响应氧浓度的能量和氨基酸代谢。
Fungi have been reported to be the dominant eukaryotic group in anoxic sub‐seafloor sediments, but how fungi subsist in the anoxic sub‐marine sedimental environment is rarely understood. Our previous study demonstrated that the fungus,Schizophyllum commune20R‐7‐F01 isolated from a ~2 km sediment below the seafloor, can grow and produce primordia in the complete absence of oxygen with enhanced production of branched‐chain amino acids (BCAAs), but the primordia cannot be developed into fruit bodies without oxygen. Here, we present the individual and synergistic effects of oxygen and BCAAs on the fruit‐body development of this strain. It was found that the fungus required a minimum oxygen concentration of 0.5% pO2to generate primordia and 1% pO2to convert primordia into mature fruit body. However, if BCAAs (20 mM) were added to the medium, the primordium could be developed into fruit body at a lower oxygen concentration up to 0.5% pO2where genesfst4andc2h2playing an important role in compensating oxygen deficiency. Moreover, under hypoxic conditions, the fungus showed an increase in mitochondrial number and initiation of auto‐phagocytosis. These findings suggest that the fruit‐body formation ofS. communemay have multiple mechanisms, including energy and amino acid metabolism in response to oxygen concentrations.