Long term effects of high fat and sucrose diets on obesity and lymphocyte proliferation in mice
Long term effects of high fat and sucrose diets on obesity and lymphocyte proliferation in mice
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DOI：
10.1007/s12603-009-0170-2
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发表时间：
2009-07-01
影响因子：
5.8
通讯作者：
Sato, K.
中科院分区：
文献类型：
--
作者：
Sato-Mito, N.;Suzui, M.;Sato, K.
为了阐明长期饲喂高脂蔗糖对C57BL/6J小鼠肥胖和免疫功能的影响，以及蔗糖替代其他碳水化合物对C57BL/6J小鼠肥胖和免疫功能的影响，对高脂、高脂加高蔗糖、高蔗糖和对照饲料喂养7个月的4组小鼠的肥胖和免疫细胞功能进行了研究。高脂、高脂加高糖饮食组小鼠体重、脂肪组织重量、血清瘦素、血糖和胰岛素水平显著高于对照组或高蔗糖饮食组，而血清可溶性瘦素受体水平显著低于对照组或高蔗糖饮食组。肥胖组、高脂组和高脂加高蔗糖组小鼠脾细胞在T细胞有丝分裂原(PHA、ConA、抗CD3抗体)和B细胞有丝分裂原(LPS)刺激下的增殖反应均显著低于对照组和高蔗糖组。然而，这些参数在高脂肪和高脂肪+高蔗糖组之间没有差异。长期喂养高脂饮食和高脂肪+高蔗糖饮食同样会导致C57BL/6J小鼠严重肥胖。高脂肪或高脂肪加高蔗糖饮食导致严重肥胖的小鼠不仅T细胞，而且B细胞的功能也可能受到损害。
To clarify the effect of prolonged feeding of a high-fat and sucrose, and to clarify the effect of sucrose instead of other carbohydrate on obesity and immunity in C57BL/6J mice.We investigated the development of obesity and immune cell function in four groups of mice fed high-fat, high-fat plus high-sucrose, high-sucrose, and control diet for 7 months.Mice fed high-fat and high-fat plus high-sucrose groups developed severe obesity. Body weight, adipose tissue weight, serum leptin, blood glucose, and insulin were significantly higher, while the level of serum soluble leptin receptor was significantly lower in mice fed high-fat and high-fat plus high-sucrose diets than in mice fed the control or high-sucrose diets. Splenocyte proliferation stimulated by T-cell mitogen (PHA, ConA, and anti-CD 3 antibody) and B-cell mitogen (LPS) was significantly lower in both obese, high-fat and high-fat plus high-sucrose groups than in control and high-sucrose groups. However, these parameters did not differ between high-fat and high-fat plus high-sucrose groups.Long-term feeding of high-fat diet and high-fat plus high-sucrose diet similarly induced severe obesity in C57BL/6J mice. Not only T-cell, but also B-cell function may be impaired in mice made severely obese by the high-fat or high-fat plus high-sucrose diets.