Determining the Volume of Additive Solution and Residual Plasma in Whole Blood Filtered and Buffy Coat Processed Red Cell Concentrates
Determining the Volume of Additive Solution and Residual Plasma in Whole Blood Filtered and Buffy Coat Processed Red Cell Concentrates
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DOI：
10.1159/000441959
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发表时间：
2016-01-01
影响因子：
2.2
通讯作者：
Acker, Jason P.
中科院分区：
文献类型：
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作者：
Jordan, Andrew;Acker, Jason P.
背景：输注浓缩红细胞（RCC）中的残留血浆与不良输血结局相关。尽管如此，对于测量残留血浆量的标准程序尚未达成共识。研究方法：使用两种分离方法：全血过滤（WBF）和血沉棕黄层（BC）/RCC过滤，测量RCC中残留血浆和添加剂溶液的体积。采用比色法测定RCC组分中甘露醇和白蛋白的浓度。使用甘露醇浓度计算添加剂溶液体积。使用两种方法计算残留血浆体积。结果如下：计算的RCC上清液体积在BC处理的组分中比在WBF处理的组分中低得多（BC = 97 +/-6ml，WBF = 109 +/-4ml; p < 0.05）。计算的添加剂溶液体积在WBF-处理的组分中大于在BC-处理的组分中（BC = 81 +/-4ml，WBF = 105 +/-2ml; p < 0.05）。根据所用计算方法，绝对残留血浆体积差异显著。结论：血浆容量计算方法之间存在差异。应努力规范残余血浆量测量方法，以准确评估残余血浆对输血结局的影响。(C)2015 S. Karger GmbH，弗赖堡
Background: Residual plasma in transfused red cell concentrates (RCCs) has been associated with adverse transfusion outcomes. Despite this, there is no consensus on the standard procedure for measuring residual plasma volume. Methods: The volumes of residual plasma and additive solution were measured in RCCs processed using two separation methods: whole blood filtration (WBF) and buffy coat (BC)/RCC filtration. The concentration of mannitol and albumin in RCC components was measured using colorimetric assays. Mannitol concentration was used to calculate additive solution volume. Residual plasma volume was calculated using two methods. Results: Calculated RCC supernatant volumes were much lower in BC-processed components compared to WBF-processed components (BC = 97 +/- 6 ml, WBF = 109 +/- 4 ml; p < 0.05). Calculated additive solution volumes were greater in WBF- than in BC-processed connponents (BC = 81 +/- 4 ml, WBF = 105 +/- 2 ml; p < 0.05). Absolute residual plasma volume varied significantly based on the calculation method used. Conclusion: Disparity between plasma volume calculation methods was observed. Efforts should be made to standardize residual plasma volume measurement methods in order to accurately assess the impact of residual plasma on transfusion outcomes. (C) 2015 S. Karger GmbH, Freiburg