Chronic exposure to environmental levels of cis-bifenthrin: Enantioselectivity and reproductive effects on zebrafish (Danio rerio)
Chronic exposure to environmental levels of cis-bifenthrin: Enantioselectivity and reproductive effects on zebrafish (Danio rerio)
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长期暴露于环境水平的顺式联苯菊酯：对斑马鱼（Danio rerio）的对映选择性和生殖影响
DOI：
10.1016/j.envpol.2019.04.089
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发表时间：
2019
影响因子：
8.9
通讯作者：
Zhou Bingsheng
中科院分区：
文献类型：
--
作者：
Xiang D;an;Zhong Linxi;Shen Shuyuan;Song Zhuoying;Zhu Guonian;Wang Mengcen;Wang Qiangwei;Zhou Bingsheng
合成拟除虫菊酯（SP）被广泛用于控制害虫，并已在水生环境中被广泛检测到，引发了人们对其对非目标生物健康风险的担忧。本研究评价了低剂量（0、20、100和500 ng/L）的顺式-BF对映体（1 S-cis-BF和1 R-cis-BF）长期（60 d）暴露是否会引起斑马鱼生殖内分泌紊乱。1 S-cis-BF对鱼类的生殖损伤作用强于1 R-对映体，表明cis-BF对鱼类生殖的对映体选择性。结果表明，暴露于顺式BF后，斑马鱼的累积产卵量显著减少。组织病理切片显示睾丸和卵巢发育迟缓是导致精子活力下降的重要原因，而对精子密度无明显影响。在雄性和雌性的性腺中检测到相对高水平的顺式BF可能直接损害配子发生。此外，与生殖内分泌途径相关的关键基因cyp 17、cyp 19 a和17 β-hsd表达的改变与性激素（E2和T）含量的显著变化密切相关，这些结果可能与雌性或/和雄性生殖细胞的性腺发育和成熟有关，这可能是一种潜在的机制。未暴露雌鼠和暴露雄鼠（UE × E）F1代胚胎质量的下降表明，雄鼠暴露对后代的不良影响更大。我们的研究结果表明，长期，低剂量暴露于cis-BF可以对映体选择性损害鱼类的生殖系统，并诱导非暴露后代的毒性相关的异常。本研究对水体中手性农药的环境风险评价具有重要意义。
Synthetic pyrethroids (SPs) are broadly used to control pests and have been widely detected in aquatic environments, prompting concern over its risk to the health of non-target organisms. The present study evaluated whether long-term (60 d) exposure to low doses (0, 20, 100, and 500 ng/L) ofcis-BF enantiomers (1S-cis-BF and 1R-cis-BF) could cause reproductive endocrine disturbance to zebrafish. Exposure to 1S-cis-BF has stronger reproductive impairment effect than 1R-enantiomer, indicating that the enantioselectivity ofcis-BF on fish reproduction. Significant decrease of cumulative spawning of zebrafish was observed as a result ofcis-BF exposure. And the retardations of testis and ovaries development found in histopathological section were suggested to be important cause for the decreased fecundity.Cis-BF decreased the total motility of sperm but did not affect sperm density. Relatively high levels ofcis-BF detected in the gonads of males and females may directly impair gametogenesis. In addition, alterations in the expression of key genes (cyp17,cyp19aand17β-hsd) associated with reproductive endocrine pathways were correlated well with the significant changes in sex hormone contents (E2 and T) and these results may relate to gonadal development and maturation of germ cells in females or/and males which were suspected to be a likely underlying mechanism. Furthermore, the reduction of quality of F1 embryo derived from the unexposed females and exposed males (UE♀ × E♂) demonstrated that male exposure had greater adverse effects on offspring. Our results indicate that long term, low dose exposure tocis-BF can enantioselectively impair the reproduction system of fish, and induce toxicity related abnormalities in non-exposed offspring. This study has important implications for environmental risk assessment of chiral pesticides that are concurrently present in aquatic systems.