THE LETHAL MYOSPHEROID GENE OF DROSOPHILA ENCODES A MEMBRANE-PROTEIN HOMOLOGOUS TO VERTEBRATE INTEGRIN BETA-SUBUNITS
THE LETHAL MYOSPHEROID GENE OF DROSOPHILA ENCODES A MEMBRANE-PROTEIN HOMOLOGOUS TO VERTEBRATE INTEGRIN BETA-SUBUNITS
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DOI：
10.1073/pnas.85.8.2633
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发表时间：
1988-04-01
影响因子：
11.1
通讯作者：
FESSLER, JH
中科院分区：
文献类型：
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作者：
MACKRELL, AJ;BLUMBERG, B;FESSLER, JH
黑腹果蝇的一种突变体携带致死性（1）肌球样突变[l（1）mys]，具有缺陷的肌肉组织和提示基底膜缺陷的表型。在携带l（1）mys的突变体中插入中断的基因组区域用于分离cDNA克隆，其序列在此呈现。该cDNA序列预测富含半胱氨酸的整合膜蛋白，其与鸡整联蛋白和人纤连蛋白受体β显示45%的序列同一性。亚基和更大的相似性超过本地化的部分。这些相似性延伸至其他脊椎动物整联蛋白β。亚基，并且我们得出结论，肌球蛋白是整联蛋白β。果蝇的亚基。这意味着一组跨膜蛋白的细胞外基质的受体，再加上肌球蛋白表型的进化保守，表明在发展过程中细胞与细胞外基质的相互作用的重要作用。
A mutant of Drosophila melangogaster carrying the lethal (1) myospheroid mutation [l(1)mys] has a defective musculature and a phenotype that suggests a defect of basement membranes. The genomic region that is interrupted by an insertion in a mutant carrying l(1)mys was used to isolate cDNA clones, and their sequences are presented here. The cDNA sequence predicts a cysteine-rich integral membrane protein that displays 45% sequence identity to chicken integrin and the human fibronectin receptor .beta. subunit and much greater similarity over localized segments. These similarities extended to other vertebrate integrin .beta. subunits, and we conclude that the myospheroid protein is an integrin .beta. subunit of Drosophila. This implies evolutionary conservation of a group of transmembrane proteins that are receptors for extracellular matrix and, coupled with the myospheroid phenotype, indicates an important role for the interaction of cells with extracellular matrix during development.