miR398 regulation in rice of the responses to abiotic and biotic stresses depends on CSD1 and CSD2 expression
miR398 regulation in rice of the responses to abiotic and biotic stresses depends on CSD1 and CSD2 expression
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DOI：
10.1071/fp10178
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发表时间：
2011-01-01
影响因子：
3
通讯作者：
Liang, Jiansheng
中科院分区：
文献类型：
--
作者：
Lu, Yuzhu;Feng, Zhen;Liang, Jiansheng
MiR 398靶向拟南芥（Arabidopsis thaliana（L.）嘿在这里，我们提供的证据表明，水稻（Oryza sativa L.）miR 398通过调节其靶基因Os-CSD 1和Os-CSD 2的表达来介导对非生物和生物胁迫的应答。本研究以水稻为材料，研究了高Cu ~（2+）、高盐、强光、甲基紫精、水分胁迫、病原菌和乙烯等胁迫对水稻的分子响应。过表达Os-miR 398和miR 398抗性形式的Os-CSD 2的水稻植物也暴露于这些胁迫。非生物胁迫和生物胁迫均显著抑制Os-miR 398的表达，从而刺激Os-CSD 1和Os-CSD 2的表达。植物激素乙烯产生了特别明显的反应。过量表达Os-miR 398的转基因水稻株系中，CSD 1和CSD-2的表达量较低，对环境胁迫更敏感。相反，过表达miR 398抗性形式的Os-CSD 2的转基因水稻品系比非转基因水稻表现出更高的耐盐性和水分胁迫性。Os-miR 398调控水稻对多种环境胁迫和乙烯胁迫的反应，并通过介导CSDs的表达和细胞内ROS水平发挥作用。
MiR398 targets two Cu or Zn superoxide dismutases (CSD1 and CSD2) in Arabidopsis thaliana (L.) Heynh. Here we provide evidence that rice (Oryza sativa L.) miR398 mediates responses to abiotic and biotic stresses through regulating the expression of its target genes, Os-CSD1 and Os-CSD2. Rice plants were exposed to various stresses, including high Cu2+, high salinity, high light, methyl viologen, water stress, pathogens and ethylene, and the molecular response was investigated. Rice plants overexpressing Os-miR398 and the miR398-resistant form of Os-CSD2 were also exposed to these stresses. Both abiotic and biotic stresses significantly inhibited Os-miR398 expression and thus stimulated the expression of Os-CSD1 and Os-CSD2. The plant hormone ethylene produced an especially marked response. Transgenic rice lines that overexpressed Os-miR398 had a lower expression of CSD1 and -2 and were more sensitive to environmental stress. Conversely, transgenic rice lines which overexpressed the miR398-resistant form of Os-CSD2 showed more tolerance to high salinity and water stress than non-transgenic rice. We conclude that Os-miR398 regulates the responses of rice to a wide range of environmental stresses and to ethylene, and exerts its role through mediating CSDs expression and cellular ROS levels.