A high-resolution map of human RNA translation
A high-resolution map of human RNA translation
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DOI：
10.1016/j.molcel.2022.06.023
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发表时间：
2022-08-04
期刊：
影响因子：
16
通讯作者：
Schafer, Sebastian
中科院分区：
文献类型：
--
作者：
Chothani, Sonia P.;Adami, Eleonora;Schafer, Sebastian
翻译的小分子开放阅读框架(SmORF)可以发挥重要的调节作用并编码微蛋白，但它们在基因组范围内的鉴定一直是一个挑战。我们确定了六种主要人类细胞类型和五种组织中的核糖体位置，并检测到7767个翻译图谱与已知蛋白质匹配的smORF。人类基因组被发现含有高度细胞类型和组织特异性的smORF，以及一个编码高度保守的氨基酸序列的子集。上游编码的单链ORF(UORF)和相应的主ORF的翻译效率主要发生在同一方向上。结合456个质谱学数据集，证实了在人类蛋白质水平上存在603个小肽，并为这些小蛋白质的亚细胞定位提供了洞察力。这项研究提供了一份来自原代人类细胞和组织的高置信度翻译的smORF的综合图谱，以提供对翻译的人类基因组的更完整的了解。
Translated small open reading frames (smORFs) can have important regulatory roles and encode microproteins, yet their genome-wide identification has been challenging. We determined the ribosome locations across six primary human cell types and five tissues and detected 7,767 smORFs with translational profiles matching those of known proteins. The human genome was found to contain highly cell-type- and tissue-specific smORFs and a subset that encodes highly conserved amino acid sequences. Changes in the translational efficiency of upstream-encoded smORFs (uORFs) and the corresponding main ORFs predominantly occur in the same direction. Integration with 456 mass-spectrometry datasets confirms the presence of 603 small peptides at the protein level in humans and provides insights into the subcellular localization of these small proteins. This study provides a comprehensive atlas of high-confidence translated smORFs derived from primary human cells and tissues in order to provide a more complete understanding of the translated human genome.