Light at night disrupts diel patterns of cytokine gene expression and endocrine profiles in zebra finch (Taeniopygia guttata)
Light at night disrupts diel patterns of cytokine gene expression and endocrine profiles in zebra finch (Taeniopygia guttata)
复制标题
DOI：
10.1038/s41598-019-51791-9
复制
发表时间：
2019-11-01
影响因子：
4.6
通讯作者：
Ashley, Noah T.
中科院分区：
文献类型：
--
作者：
Mishra, Ila;Knerr, Reinhard M.;Ashley, Noah T.
暴露于光污染的增加通过在细胞和组织水平上的日常节律失调而扰乱生理过程。夜间人造光对人体免疫特性的影响目前尚不清楚。因此，我们在斑胸草雀（Taeniopygia guttata）中测试了ALAN对细胞因子基因表达的昼夜模式以及参与免疫调节的关键激素的影响。在24小时内的6个时间点，测量暴露于12小时光照下的斑胸草雀的大脑（nidopallium、海马和下丘脑）和外周组织（肝脏、脾脏和脂肪）中循环褪黑激素和皮质酮的mRNA表达水平，包括12小时黑暗（LD）、夜间昏暗（DLAN）或恒定强光（LLbright）。褪黑激素和皮质酮浓度在LD下有显著的节律性，而在LLbright和DLAN下没有。除了下丘脑和肝脏中的IL-6外，编码细胞因子的基因在LD下显示出组织特异性的昼夜节律，并且随着暴露于LLbright而丢失。与LLbright相比，尽管存在显著的波形改变，但DLAN对某些昼夜节律的持续影响较小。这些结果强调了免疫效应物生物合成的昼夜节律调节，并暗示了日常免疫和内分泌模式对ALAN的易感性。
Increased exposure to light pollution perturbs physiological processes through misalignment of daily rhythms at the cellular and tissue levels. Effects of artificial light-at-night (ALAN) on diel properties of immunity are currently unknown. We therefore tested the effects of ALAN on diel patterns of cytokine gene expression, as well as key hormones involved with the regulation of immunity, in zebra finches (Taeniopygia guttata). Circulating melatonin and corticosterone, and mRNA expression levels of pro(IL-1 beta, IL-6) and anti-inflammatory (IL-10) cytokines were measured at six time points across 24-h day in brain (nidopallium, hippocampus, and hypothalamus) and peripheral tissues (liver, spleen, and fat) of zebra finches exposed to 12 h light:12 h darkness (LD), dim light-at-night (DLAN) or constant bright light (LLbright). Melatonin and corticosterone concentrations were significantly rhythmic under LD, but not under LLbright and DLAN. Genes coding for cytokines showed tissue-specific diurnal rhythms under LD and were lost with exposure to LLbright, except IL-6 in hypothalamus and liver. In comparison to LLbright, effects of DLAN were less adverse with persistence of some diurnal rhythms, albeit with significant waveform alterations. These results underscore the circadian regulation of biosynthesis of immune effectors and imply the susceptibility of daily immune and endocrine patterns to ALAN.