Protein Z and protein Z-dependent protease inhibitor and renal tubules.
Protein Z and protein Z-dependent protease inhibitor and renal tubules.
复制标题
蛋白 Z 和蛋白 Z 依赖性蛋白酶抑制剂和肾小管。
DOI：
10.1160/th09-02-0131
复制
发表时间：
2010
影响因子：
6.7
通讯作者：
Tu,Y
中科院分区：
文献类型：
--
作者：
BrozeJr,GJ;Tu,Y
Protein Z (PZ) is a vitamin K-dependent, plasma protein that serves as a cofactor for the inhibition of factor Xa by protein Z-dependent protease inhibitor (ZPI) in the presence of calcium ions and phospholipids. ZPI is a member of the serpin superfamily of proteinase inhibitors and not only inhibits factor Xa in a PZ-dependent fashion, but also inhibits factor XIa in the absence of cofactors. Both PZ and ZPI are thought to be produced predominantly by the liver (1).To assess other potential sources of PZ besides the liver, tissue northern analysis was performed and showed substantial PZ mRNA expression (~ 1.4 kb) in the kidney (* Fig. 1A). Subsequent immunohistochemistry studies using two monoclonal anti-PZ antibodies (Mab 2048, Mab 2306. BF12. 2)(2) demonstrated PZ staining in the renal distal and collecting tubules based on tubular morphology and location in paraffin and frozen sections of the renal cortex (not shown) and the renal medulla (Fig. 1C). ZPI was also detected in kidney tubules by immunohistochemical analysis using two monoclonal antibodies (Mab 4242.2, Mab 4336.1 E5)(3) in the same location as PZ (Fig. 1D). As Northern analy-
