Effect of protein on the dissolution of HA coatings.
Effect of protein on the dissolution of HA coatings.
复制标题
DOI：
10.1016/s0142-9612(99)00183-0
复制
发表时间：
2000-02
期刊：
影响因子：
14
通讯作者：
S. A. Bender;J. Bumgardner;Roach;K. Bessho;J. Ong
中科院分区：
文献类型：
--
作者：
S. A. Bender;J. Bumgardner;Roach;K. Bessho;J. Ong
需要研究羟基磷灰石 (HA) 在存在和不存在蛋白质的情况下的溶解行为，以便充分了解细胞对 HA 表面的初始反应。在这项研究中，在蛋白质研究之前，使用 X 射线衍射、X 射线光电子能谱和傅里叶变换红外光谱 (FTIR) 对 HA 涂层进行了表征。还进行了纤连蛋白和白蛋白吸附研究。测量了在存在和不存在白蛋白的情况下释放的钙和磷。每天测量溶液的pH值。从材料表征来看，涂层呈现HA型结构，表面有微量钠。 FTIR 表明涂层上可能存在碳酸盐。从吸附研究来看，白蛋白的吸附量（0.052±0.005μg/mm2）统计上高于HA表面的纤连蛋白吸附量（0.035±0.002μg/mm2）。火焰原子吸收表明，与不存在蛋白质的涂层相比，与蛋白质一起孵育的 HA 涂层最初释放的钙离子明显更高。然而，孵育 7 天后，在存在和不存在蛋白质的情况下，HA 涂层之间没有观察到钙离子释放的显着差异。 HA 涂层上的磷溶解并未受到蛋白质存在的显着影响。因此，这项研究表明，HA 涂层中钙离子的初始溶解特性取决于介质。
The dissolution behavior of hydroxyapatite (HA) in the presence and absence of protein needs to be investigated in order to fully understand the initial cellular response to HA surfaces. In this study, HA coatings were characterized using X-ray diffraction, X-ray photoelectron spectroscopy, and Fourier transform infrared spectroscopy (FTIR) prior to protein study. Fibronectin and albumin adsorption study were also performed. Calcium and phosphorus released in the presence and absence of albumin were measured. pH of the solution was measured daily. From the materials characterization, it was observed that the coatings exhibit a HA-type structure, with traces of sodium on the surface. FTIR indicated the possible presence of carbonates on the coatings. From the adsorption study, the amount of albumin adsorbed (0.052±0.005μg/mm2) was statistically higher than the amount of fibronectin adsorbed on HA surfaces (0.035±0.002μg/mm2). Flame atomic absorption indicated a significantly higher calcium ions released initially for HA coatings incubated with proteins as compared to coatings in the absence of proteins. However, after 7 days incubation, no significant difference in calcium ions release was observed between the HA coatings in the presence and absence of proteins. Phosphorus dissolution on HA coatings was not significantly affected by the presence of proteins. Thus, it was suggested from this study that the initial dissolution properties of calcium ions from HA coatings was dependent on the media.