Dietary seaweed (Laminaria) and mammary carcinogenesis in rats.
Dietary seaweed (Laminaria) and mammary carcinogenesis in rats.
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发表时间：
1984-07
期刊：
影响因子：
11.2
通讯作者：
J. Teas;M. Harbison;R. Gelman
中科院分区：
文献类型：
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作者：
J. Teas;M. Harbison;R. Gelman
为了测试膳食海藻体内潜在的抗肿瘤作用，我们用致癌物7，12-二甲基苯并(A)菲诱发雌性SD大鼠乳腺肿瘤。将21只1日龄大鼠(n=108)分为两组。对照组喂以标准的半纯饲料，实验大鼠喂以5%的棕色海带海带代替5%的海带。55日龄时，每只大鼠灌胃给予5 mg 7，12-二甲基苯并(A)蒽。对大鼠进行乳腺肿瘤触诊，每周称重26周。然后对所有大鼠进行完整的尸检。海藻饮食没有改变身体解剖时的体重增加或身体器官的重量。实验大鼠出现肿瘤的时间明显延迟(p=0.007)；实验大鼠肿瘤发生的中位数时间为19周，对照组为11周。在乳腺癌荷瘤动物中，实验大鼠每只患腺癌的数量较少(p<0.05)。组织学证实患有腺癌的实验大鼠的数量总体上也减少了13%(对照组大鼠减少了76%，而实验大鼠减少了63%)。海带的成分多种多样，可能解释了观察到的乳腺肿瘤生长的差异，其中包括硫酸多糖岩藻糖胶。与笼子下面四排的大鼠相比，笼子顶排的大鼠肿瘤发生的时间明显延迟(p=0.01)。在每一排中，喂海藻的大鼠比对照组的大鼠有更长的肿瘤发生时间。
To test the potential in vivo antitumor effect of dietary seaweed, we induced mammary tumors in female Sprague-Dawley rats with the carcinogen 7,12-dimethylbenz(a)anthracene. Twenty-one-day-old rats (n = 108) were divided into two groups. Controls were fed a standard semipurified diet, and experimental rats received the control diet with 5% Laminaria, a brown seaweed, replacing 5% alphacel . At 55 days of age, each rat received 5 mg 7,12-dimethylbenz(a)anthracene intragastrically. Rats were palpated for mammary tumors and weighed weekly for 26 weeks. Complete autopsies were then done on all rats. The seaweed diet did not alter weight gain or weights of body organs at autopsy. Experimental rats had a significant delay in the time to tumor (p = 0.007); median time until tumor was 19 weeks in experimental rats and 11 weeks in control animals. Among mammary adenocarcinoma tumor-bearing animals, experimental rats had fewer adenocarcinomas/individual (p less than 0.05). There was also an overall 13% reduction in the number of experimental rats with histologically confirmed adenocarcinomas (76% among the control rats compared to 63% among the experimental rats). Components of Laminaria which might account for the observed difference in mammary tumor growth are varied and include the sulfated polysaccharide fucoidan . Rats in the top row of cages had a significant (p = 0.01) delay in time to tumor compared to rats in the lower four rows. In each row, the seaweed-fed rats had a longer time to tumor than did the control rats.