Bioremediation of 2,4,6-Trinitrotoluene by Bacterial Nitroreductase Expressing Transgenic
Bioremediation of 2,4,6-Trinitrotoluene by Bacterial Nitroreductase Expressing Transgenic
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2020
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2，4，6-三硝基甲苯(TNT)是地雷、军事和矿业中最常用的硝基芳香族炸药之一。本文以6-氨基己硫醇(AHT)为识别分子，用表面增强拉曼光谱(SERS)对TNT进行了快速、选择性的识别。首先，AHT与TNT形成了Meisenheimer络合物，在紫外可见光谱中，在500 nm附近出现了新的谱带，呈粉红色。溶液拉曼光谱研究还证实了AHT分子在2800-3000 cm-1之间振动伸缩的变化，从而支持了AHT：TNT络合物的形成。对于表面增强拉曼光谱分析，在金纳米结构(AUNS)SERS衬底上形成了AHT的自组装单分子膜(SAM)，以便选择性地将TNT捕获到表面。在AHTSAM修饰表面上进行了电化学脱附和X射线光电子研究，以考察是否存在具有合适取向的游离胺基团以形成络合物。进一步探索了AHT与丁硫醇(BT)的混合单层体系，以提高AHT：TNT络合物的形成效率。使用9：1的AHT：BT混合单层膜，实现了100fM TNT的极低检出限(LOD)。新方法对2，4-二硝基甲苯和苦味酸具有较高的选择性。最后，通过对302 Pm TNT的提取和SERS检测，验证了实际样品分析的可行性。用于生物分析的可展开光谱工具和有效的直接表面增强拉曼散射，
2,4,6-trinitrotoluene (TNT) is one of the most commonly used nitro aromatic explosives in landmine, military and mining industry. This article demonstrates rapid and selective identification of TNT by surface-enhanced Raman spectroscopy (SERS) using 6-aminohexanethiol (AHT) as a new recognition molecule. First, Meisenheimer complex formation between AHT and TNT is confirmed by the development of pink colour and appearance of new band around 500 nm in UV-visible spectrum. Solution Raman spectroscopy study also supported the AHT:TNT complex formation by demonstrating changes in the vibrational stretching of AHT molecule between 2800-3000 cm -1 . For surface enhanced Raman spectroscopy analysis, a self-assembled monolayer (SAM) of AHT is formed over the gold nanostructure (AuNS) SERS substrate in order to selectively capture TNT onto the surface. Electrochemical desorption and X-ray photoelectron studies are performed over AHT SAM modified surface to examine the presence of free amine groups with appropriate orientation for complex formation. Further, AHT and butanethiol (BT) mixed monolayer system is explored to improve the AHT:TNT complex formation efficiency. Using a 9:1 AHT:BT mixed monolayer, a very low detection limit (LOD) of 100 fM TNT was realized. The new method delivers high selectivity towards TNT over 2,4 DNT and picric acid. Finally, real sample analysis is demonstrated by the extraction and SERS detection of 302 pM of TNT from spiked. deployable spectroscopic tools for and effective direct surface-enhanced Raman scattering for bioassays,