CYTOCHROME P4502E1 (CYP2E1) GENETIC-POLYMORPHISM IN A CASE-CONTROL STUDY OF GASTRIC-CANCER AND LIVER-DISEASE
CYTOCHROME P4502E1 (CYP2E1) GENETIC-POLYMORPHISM IN A CASE-CONTROL STUDY OF GASTRIC-CANCER AND LIVER-DISEASE
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DOI：
10.1097/00008571-199512001-00016
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发表时间：
1995-01-01
期刊：
影响因子：
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通讯作者：
SHIELDS, PG
中科院分区：
文献类型：
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作者：
KATO, S;ONDA, M;SHIELDS, PG
细胞色素P4502 E1（CYP 2 E1）激活致癌的N-亚硝胺、苯、尿烷和其他低分子量化合物，该酶也可被乙醇诱导，并代谢酒精。使用Rsa I限制性内切酶的限制性片段长度多态性（RFLP）已在CYP 2 E1转录调控区被鉴定;最近的研究表明，这种多态性可能影响基因表达。我们调查了日本人群中Rsa I RFLP的频率与胃癌和肝病易感性的关系。在150例胃癌、16例肝细胞癌、48例肝硬化和203例良性胃病（对照）患者中测定了该多态性的频率。这项初步研究表明，在所有受试者中，特定基因型与胃癌无关（杂合子的比值比= 1.04，95%CI = 0.74-3.08，纯合子的比值比= 0.57，95%CI = 0.22-1.50），为了进一步证实这种缺乏关联，另外，Rsa I RFLP与肝细胞癌无相关性（p = 0.911），但在非病毒相关性肝硬化患者和对照组之间存在差异。因此，这种多态性可能与日本人群中的乙醇代谢和相应的肝脏疾病有关。
Cytochrome P4502E1 (CYP2E1) activates carcinogenic N-nitrosamines, benzene, urethane and other low molecular weight compounds, This enzyme is also inducible by ethanol, and metabolizes alcohol. A restriction fragment length polymorphism (RFLP) using the Rsa I restriction enzyme has been identified in the CYP2E1 transcription regulatory region; recent studies suggest that this polymorphism may affect gene expression. We investigated the frequency of the Rsa I RFLP in a Japanese population in relation to gastric cancer and liver disease susceptibility. The frequency of this polymorphism was determined in 150 gastric cancer, 16 hepatocellular cancer, 48 liver cirrhosis and 203 benign gastric disease (controls) patients. This preliminary study shows no association of the specific genotype with gastric cancer in all subjects (odds ratio = 1.04, 95% CI = 0.74-3.08 for the heterozygote and 0.57, 95% CI = 0.22-1.50 for the homozygous rare allele, respectively), To further confirm this lack of association, an age and gender matched case-control study should be performed.Separately, there was no association of the Rsa I RFLP with hepatocellular carcinoma (p = 0.911), but there was a suggested difference between the non-viral associated liver cirrhosis patients and control patients. Thus, this polymorphism may be related to ethanol metabolism and consequential liver diseases in a Japanese population.