GABA (A) receptor subunits RNA expression in mice peritoneal macrophages modulate their IL-6/IL-12 production
GABA (A) receptor subunits RNA expression in mice peritoneal macrophages modulate their IL-6/IL-12 production
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DOI：
10.1016/j.jneuroim.2007.05.013
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发表时间：
2007-08-01
影响因子：
3.3
通讯作者：
Garcia-Tamayo, Fernando
中科院分区：
文献类型：
--
作者：
Reyes-Garcia, Maria Guadalupe;Hernandez-Hernandez, Francisca;Garcia-Tamayo, Fernando
探讨γ-氨基丁酸A受体（GABAAR）在小鼠巨噬细胞中的表达和功能。逆转录-聚合酶链反应显示巨噬细胞表达α 1、α 2、β 3和δ亚基的GABA（A）R RNA，但不表达α 5、β 1、β 2和γ 3亚基。共聚焦显微镜观察ot I亚基的表达。在培养液中加入GABA后，LPS刺激的巨噬细胞在体外产生IL-6和IL- 12的能力显著降低。这些结果表明，BALB/c小鼠腹腔巨噬细胞表达GABAAR亚基的功能子集。(c)2007 Elsevier B. V.保留所有权利。
The expression and functionality of the gamma aminobutyric acid A receptor (GABAAR) in mice macrophages was explored. Reverse Transcriptase-Polymerase Chain Reaction showed that macrophages express the GABA(A)R RNA for the alpha 1, alpha 2, beta 3 and delta subunits, but not for the alpha 5, beta 1, beta 2 and gamma 3 subunits. The expression of ot I subunit was also revealed by confocal microscopy. LPS-stimulated macrophages significantly reduced their in vitro production of IL-6 and IL- 12 after GABA adding to the culture medium. These results showed that BALB/c mice peritoneal macrophages express a functional subset of GABAAR subunits. (c) 2007 Elsevier B.V. All rights reserved.