NIRS Measurement of O2 Dynamics in Contracting Blood and Buffer Perfused Hindlimb Muscle
NIRS Measurement of O2 Dynamics in Contracting Blood and Buffer Perfused Hindlimb Muscle
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DOI：
10.1007/978-1-4419-1241-1_46
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发表时间：
2010-01-01
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影响因子：
--
通讯作者：
Jue, Thomas
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文献类型：
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作者：
Masuda, Kazumi;Takakura, Hisashi;Jue, Thomas
为了获得肌红蛋白在肌肉收缩过程中释放O2的证据，我们建立了缓冲液灌注的后肢大鼠模型，并应用近红外光谱技术检测肌肉收缩过程中组织脱氧的动力学。通过电刺激器在不同的次最大水平下以1 Hz的频率诱发后肢肌肉抽搐收缩期间监测NIRS信号。后肢灌注通过Krebs Bicycle缓冲液灌注进行。即使在无Hb收缩下，NIRS仍然检测到强信号。脱氧信号（A[脱氧]）在收缩开始时逐渐增加，并在血液和缓冲液灌注条件下达到平台。然而，随着张力的增加，稳定状态下A[脱氧]的振幅继续显著增加。在缓冲液灌注和血液灌注条件下A[脱氧]水平的张力匹配比较表明Mb可以对NIRS信号贡献约50%。这些结果阐明了Mb对NIRS信号的贡献，并显示随着工作负荷的增加，细胞内PO（2）下降。
In order to obtain evidence that Mb releases 02 during muscle contraction, we have set up a buffer-perfused hindlimb rat model and applied NIRS to detect the dynamics of tissue deoxygenation during contraction. The NIRS signal was monitored on hindlimb muscle during twitch contractions at 1 Hz, evoked via electrostimulator at different submaximal levels. The hindlimb perfusion was carried out by perfusion of Krebs Bicarbonate buffer. The NIRS still detected a strong signal even under Hb-free contractions. The deoxygenation signal (A[deoxy]) was progressively increased at onset of the contraction and reached the plateau under both blood- and buffer-perfused conditions. However, the amplitude of A[deoxy] during steady state continued to significantly increase as tension increased. The tension-matched comparison of the A[deoxy] level under buffer-perfused and blood perfused conditions indicate that Mb can contribute approximately 50% to the NIRS signal. These results clarify the Mb contribution to the NIRS signal and show a falling intracellular PO(2) as workload increases.