Validation of a set of informative simple sequence repeats markers for variety identification in Pak-choi (Brassica rapa L. ssp chinensis var. communis)
Validation of a set of informative simple sequence repeats markers for variety identification in Pak-choi (Brassica rapa L. ssp chinensis var. communis)
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验证一组用于小白菜（Brassica rapa L. ssp chinensis var. communis）品种鉴定的信息丰富的简单序列重复标记
DOI：
10.1111/pbr.12483
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发表时间：
2017
期刊：
影响因子：
2
通讯作者：
Yu Yangjun
中科院分区：
文献类型：
--
作者：
Su Tongbing;Zhao Xiuyun;Sui Guanglei;Yu Shuancang;Zhang Fenglan;Wang Weihong;Zhang Deshuang;Yu Yangjun
本研究开发了21个用于小白菜品种鉴定的SSR核心标记。从2129个SSR标记中初步筛选出74个多态性和重复性较高的SSR标记。以45个自交系的74个SSR聚类图为标准，筛选出21个核心SSR。利用该核心SSR标记对45个自交系进行了检测，并对102个商品品种进行了验证。我们还为每个核心SSR构建了一个分子阶梯作为参考标准。对102个品种的SSR多态性分析表明，每个标记产生2-3个等位基因（平均2.33），多态性信息含量为0.01 ~ 0.56（平均0.31）。Shannon信息指数、观测杂合度、期望杂合度和Wright固定指数的平均值分别为0.59、0.43、0.38和-0.09。此外，102个品种的21个SSR分类与传统的基于形态学的分类一致。该核心SSR组代表了小白菜遗传变异分析的有效工具。
A core set of 21 simple sequence repeats (SSR) markers was developed for Pak‐choi (Brassicarapassp.chinensisvar.communis) variety identification. We initially selected 74 SSR markers which exhibited high polymorphism and reproducibility in SSR detection from 2129 SSRs. Using the 74 SSR‐based dendrogram for 45 inbred lines as calibration, 21 core SSRs were selected out. The utility of this core set SSRs was firstly tested in 45 inbred lines and finally verified in 102 commercial varieties. We also constructed a molecular ladder for each core SSR as a reference standard. Diversity analysis of this core SSR panel in 102 varieties demonstrated that each marker generates 2–3 alleles (averaged 2.33), with polymorphism information content values ranging from 0.01 to 0.56 (averaged 0.31). The averaged values of Shannon information index, observed heterozygosity, expected heterozygosity and Wright's fixation index were 0.59, 0.43, 0.38 and −0.09, respectively. Furthermore, the 21 SSR‐based classifications for 102 varieties were consistent with traditional classification based on morphology. This core SSR panel represents an effective tool for genetic variation analysis in Pak‐choi.