Distribution of MAP2 in dendritic spines and its colocalization with actin. An immunogold electron-microscope study.
Distribution of MAP2 in dendritic spines and its colocalization with actin. An immunogold electron-microscope study.
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MAP2 在树突棘中的分布及其与肌动蛋白的共定位。
DOI：
10.1007/bf00225592
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发表时间：
1989
影响因子：
3.6
通讯作者：
Fifkova,E
中科院分区：
文献类型：
--
作者：
Morales,M;Fifkova,E
免疫金电镜检测MAP2和肌动蛋白在视觉皮层、小脑皮层、齿状筋膜和海马树突棘中的分布。通过这种方法，我们已经证实了MAP2在树突棘中的存在，并确定了棘腔内显示MAP2免疫反应性的亚结构。MAP2免疫标记主要与单克隆抗肌动蛋白抗体反应的纤维相关。此外，通过免疫金双标记，我们将MAP2与肌动蛋白共定位在脊柱器官内膜、光滑内质网和突触后密度上。轴突和轴突末端几乎没有标记。这些结果表明MAP2是树突棘中的肌动蛋白相关蛋白。因此，MAP2可能组织脊柱中的肌动蛋白丝，并赋予脊柱的肌动蛋白网络动态特性，这是突触可塑性所必需的。
The distribution of MAP2 and actin in dendritic spines of the visual and cerebellar cortices, dentate fascia, and hippocampus was determined by using immunogold electron microscopy. By this approach, we have confirmed the presence of MAP2 in dendritic spines and identified substructures within the spine compartment showing MAP2 immunoreactivity. MAP2 immunolabeling was mainly associated with filaments which reacted with a monoclonal anti-actin antibody. Also, by immunogold double-labeling we colocalized MAP2 with actin on the endomembranes of the spine apparatus, smooth endoplasmic reticulum, and in the postsynaptic density. Labeling was nearly absent in axons and axonal terminals. These results indicate that MAP2 is an actin-associated protein in dendritic spines. Thus, MAP2 may organize actin filaments in the spine and endow the actin network of the spine with dynamic properties that are necessary for synaptic plasticity.