DETERMINATION OF DELTA-AMINOLAEVULIC ACID
DETERMINATION OF DELTA-AMINOLAEVULIC ACID
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DOI：
10.1042/bj0640101
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发表时间：
1956-01-01
影响因子：
4.1
通讯作者：
SHUSTER, L
中科院分区：
文献类型：
--
作者：
SHUSTER, L
描述了两种用于测定δ-氨基乙酰丙酸的比色方法。两者都对 0.05 摩尔敏感。一种方法是基于与乙酰乙酸乙酯在NaOH中缩合形成产物，该产物用艾利希试剂（对二甲氨基苯甲醛）呈现红色。另一种方法是 Jaffe 苦味酸盐反应的改进，依赖于氨基乙酰丙酮酸的碱性苦味酸盐络合物与 HCl 的反应，产生橙红色。两种方法都描述了不同因素对生物材料的测定、特异性和应用的影响。
Two colorimetric methods are described for the determination of [delta]-aminolaevulic acid. Both are sensitive to 0.05 mole. One method is based on condensation with ethyl acetoacetate in 2[image] NaOH to form a product which gives a red color with Ehrlich''s reagent (p-dimethylaminobenzaldehyde). The other method is a modification of the Jaffe picrate reaction and depends on the reaction of an alkaline picrate complex of aminolaevulic acid with HC1 to give an orange-red color. The effects of varying different factors in the determination, specificity and application to biological materials are described for both methods.