Skeletal muscle lipid content and oxidative enzyme activity in relation to muscle fiber type in type 2 diabetes and obesity
Skeletal muscle lipid content and oxidative enzyme activity in relation to muscle fiber type in type 2 diabetes and obesity
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DOI：
10.2337/diabetes.50.4.817
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发表时间：
2001-04-01
期刊：
影响因子：
7.7
通讯作者：
Kelley, DE
中科院分区：
文献类型：
--
作者：
He, J;Watkins, S;Kelley, DE
在肥胖症和2型糖尿病中，已经观察到骨骼肌具有降低的氧化酶活性、增加的糖酵解活性和增加的脂质含量。这些代谢特征与骨骼肌的胰岛素抵抗相关，并且是与肌纤维类型潜在相关的因素。本研究旨在研究肥胖和2型糖尿病受试者与瘦型健康志愿者相比，肌纤维类型与氧化酶活性、糖酵解酶活性和肌肉脂质含量的相互作用。采用单纤维分析方法，对22名瘦型、20名肥胖型和20名2型糖尿病受试者的股外侧肌进行经皮活检（年龄分别为35 +/- 1、42 +/- 2和52 +/-岁），肥胖和糖尿病受试者的BMI值相似（分别为23.7 +/- 0.7、33.2 +/- 0.8和31.8 +/- 0.8 kg/m2）。氧化酶活性的顺序为I型> IIa型> IIb型，但在各型肌纤维中，肥胖和2型糖尿病患者骨骼肌的氧化酶活性均低于瘦型患者（P < 0.01）。肌肉脂质含量与纤维类型的关系相似，在每种纤维类型中，肥胖和2型糖尿病受试者的肌肉脂质含量较高（P < 0.01）。总之，基于单纤维分析，肥胖和2型糖尿病受试者的骨骼肌表现出与纤维类型无关的氧化酶活性紊乱和脂质含量增加。
In obesity and type 2 diabetes, skeletal muscle has been observed to have a reduced oxidative enzyme activity, increased glycolytic activity, and increased Lipid content. These metabolic characteristics are related to insulin resistance of skeletal muscle and are factors potentially related to muscle fiber type. The current study was undertaken to examine the interactions of muscle fiber type in relation to oxidative enzyme activity, glycolytic enzyme activity, and muscle Lipid content in obese and type 2 diabetic subjects compared with lean healthy volunteers. The method of single-fiber analysis was used on vastus lateralis muscle obtained by percutaneous biopsy from 22 lean, 20 obese, and 20 type 2 diabetic subjects (ages 35 +/- 1, 42 +/- 2, and 52 +/- years, respectively), with values for BMI that were similar in obese and diabetic subjects (23.7 +/- 0.7, 33.2 +/- 0.8, and 31.8 +/- 0.8 kg/m(2), respectively). Oxidative enzyme activity followed the order of type I > type IIa > type IIb, but within each fiber type, skeletal muscle from obese and type 2 diabetic subjects had lower oxidative enzyme activity than muscle from lean subjects (P < 0.01). Muscle lipid content followed a similar pattern in relation to fiber type, and within each fiber type, muscle from obese and type 2 diabetic subjects had greater Lipid content (P < 0.01). In summary, based on single-fiber analysis, skeletal muscle in obese and type 2 diabetic subjects mani-fests disturbances of oxidative enzyme activity and increased lipid content that are independent of the effect of fiber type.