TARGET IMMUNITY OF MU-TRANSPOSITION REFLECTS A DIFFERENTIAL DISTRIBUTION OF MU-B-PROTEIN
TARGET IMMUNITY OF MU-TRANSPOSITION REFLECTS A DIFFERENTIAL DISTRIBUTION OF MU-B-PROTEIN
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DOI：
10.1016/0092-8674(88)90387-x
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发表时间：
1988-04-22
期刊：
影响因子：
64.5
通讯作者：
MIZUUCHI, K
中科院分区：
文献类型：
--
作者：
ADZUMA, K;MIZUUCHI, K
携带Mu末端DNA序列的DNA分子是Mu DNA链转移反应中的不良靶标，这种现象被称为“靶标免疫”。“我们发现Mu B蛋白通过与靶DNA结合来刺激分子间链转移。我们的研究结果表明，Mu B蛋白在“免疫”和“非免疫”DNA分子之间的差异分布是负责靶免疫;在Mu A蛋白和ATP的存在下，Mu B蛋白优先从免疫DNA分子解离。ATP的水解涉及在Mu A蛋白存在的情况下建立Mu B蛋白在免疫和非免疫DNA分子之间的差异分布;不可水解的ATP-γ S可以支持有效的链转移反应，即使是在与ATP反应中免疫的靶DNA。
A DNA molecule carrying Mu end DNA sequence(s) is a poor target in the Mu DNA strand-transfer reaction, a phenomenon which is referred to as "target immunity." We find that Mu B protein stimulates intermolecular strand-transfer by binding to the target DNA. Our results show that a differential distribution of Mu B protein between "immune" and "non-immune" DNA molecules is responsible for target immunity; in the presence of Mu A protein and ATP, Mu B protein dissociates preferentially from immune DNA molecules. Hydrolysis of ATP is implicated in establishing the differential distribution of Mu B protein between immune and non-immune DNA molecules in the presence of Mu A protein; nonhydrolyzable ATP-.gamma.S can support an efficient strand-transfer reaction even with a target DNA that is immune in a reaction with ATP.