Physicochemical properties of starches and expression and activity of starch biosynthesis-related genes in sweet potatoes
Physicochemical properties of starches and expression and activity of starch biosynthesis-related genes in sweet potatoes
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DOI：
10.1016/j.foodchem.2015.12.053
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发表时间：
2016-05-15
期刊：
影响因子：
8.8
通讯作者：
Lin, Kuan H.
中科院分区：
文献类型：
--
作者：
Lai, Yung C.;Wang, Shu Y.;Lin, Kuan H.
本文研究了6个不同淀粉成分和结构的甘薯品种淀粉的功能特性，以期确定工业用途的淀粉来源。高直链淀粉（AM）的台南18 （TNN18）具有高的挫折和破裂粘度，85℃时水溶性高，65℃时溶胀体积小，硬度和粘附性高；相反，台农31号（TNG31）的低am淀粉具有相反的特征。7个淀粉生物合成相关基因在TNN18和TNG31中高表达，其中GBSS、SS、SBEII、ISA和AGPase；然而，DBE和SBE的转录水平极低。GBSS和SS活性反映了tnn31和TNN18中GBSS和SS mRNA的丰度，而TNN18中AGPase、GBSS、SS和SBE的表达显著增加了AM的含量。随着AP含量的增加，DBE的表达和活性对TNG31有显著影响。(C) 2015 Elsevier Ltd.版权所有。
The functional properties of starches from six sweet potato varieties containing various starch components and structures were studied in an attempt to identify starch sources for industrial uses. Tainan 18 (TNN18) with high-amylose (AM) starch exhibited high setback and breakdown viscosities, high water solubility at 85 degrees C but low swelling volume at 65 degrees C, and high hardness and adhesiveness; in contrast, the low-AM starch of Tainung 31 (TNG31) had opposite characteristics. Seven genes related to starch biosynthesis were tested, and GBSS, SS, SBEII, ISA, and AGPase were highly expressed in TNN18 and TNG31; however, transcript levels in DBE and SBE were extremely low. GBSS and SS activity reflected the abundance of GBSS and SS mRNA in TNG31 and TNN18, and expression of AGPase, GBSS, SS, and SBE in TNN18 substantially increased content of AM. The expression and activity of DBE had a significant effect on TNG31 with increased AP content. (C) 2015 Elsevier Ltd. All rights reserved.