Surface modification with E-cadherin fusion protein for mesenchymal stem cell culture
Surface modification with E-cadherin fusion protein for mesenchymal stem cell culture
复制标题
E-钙粘蛋白融合蛋白表面修饰用于间充质干细胞培养
DOI：
10.1039/c6tb00765a
复制
发表时间：
2016
影响因子：
7
通讯作者：
Jun Yang
中科院分区：
文献类型：
--
作者：
Yan Zhang;Hongli Mao;Mengyuan Qian;Feifei Hu;Lei Cao;Ke Xu;Qizhi Shuai;Chao Gao;Ren Lang;Toshihiro Akaike;Jun Yang
为了在不影响人骨髓间充质干细胞（hMSCs）固有生物学特性的前提下有效扩增hMSCs，制备了人E-钙粘蛋白（E-cadherin）胞外区与免疫球蛋白G Fc区的融合蛋白（hE-cad-Fc），并将其作为MSC培养表面修饰的仿生基质。结果表明，hE-cad-Fc修饰的聚苯乙烯表面上培养的细胞表现出改善的增殖和旁分泌功能，与未修饰的和胶原蛋白修饰的聚苯乙烯表面上培养的细胞相比。同时，hE-cad-Fc修饰的表面即使在血清剥夺条件下也能有效抑制细胞凋亡。hE-cad-Fc不仅能上调β-catenin在MSCs中的表达，刺激E-cadherin/β-catenin的细胞膜复合物，而且能有效激活细胞内信号如EGFR、AKT和ERK磷酸化。因此，hE-cad-Fc似乎是生物表面修饰和干细胞培养的有希望的候选者。
To effectively expand human mesenchymal stem cells (hMSCs) in vitro without affecting their innate biological properties, a fusion protein (hE-cad-Fc) consisting of a human E-cadherin extracellular domain and an immunoglobulin G Fc region was fabricated and used as a biomimetic matrix for MSC culture surface modification. The results showed that cells cultured on hE-cad-Fc-modified polystyrene surfaces exhibited improved proliferation and paracrine functions compared with cells cultured on unmodified and collagen-modified polystyrene surfaces. Meanwhile, surfaces modified with hE-cad-Fc effectively inhibited cell apoptosis even under the serum deprivation conditions. Additionally, the hE-cad-Fc not only up-regulated the expression of β-catenin in MSCs and stimulated the cellular membrane complex of E-cadherin/β-catenin, but also effectively activated the intracellular signals such as EGFR, AKT and ERK phosphorylation. Therefore, hE-cad-Fc appeared to be a promising candidate for biological surface modification and stem cell culture.