Molecular characterization and functional analysis of Bxy-octr-1 in Bursaphelenchus xylophilus
Molecular characterization and functional analysis of Bxy-octr-1 in Bursaphelenchus xylophilus
复制标题
松材线虫 Bxy-octr-1 的分子表征和功能分析
DOI：
10.1016/j.gene.2022.146350
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发表时间：
2022-02-24
期刊：
影响因子：
3.5
通讯作者：
Hu, Jiafu
中科院分区：
文献类型：
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作者：
Wang, Jinghan;Liu, Huilin;Hu, Jiafu
松材线虫（Bursaphelenchusxylophilus）是一种入侵性植物寄生线虫，在世界范围内引起松树枯萎病，造成巨大的经济损失。G蛋白偶联受体（GPCRs）在动物的交配和繁殖行为中起着重要作用。章鱼胺（octopamine，OA）是无脊椎动物中特有的生物胺，可通过与特异性GPCR结合来调节多种生理和行为反应。这些特定的GPCR也被称为章鱼胺受体（OAR），octr-1就是其中之一。然而，Bxy-octr-1在B中未知。木生植物因此，我们研究了Bxy-octr-1的表达模式和生物学功能。生物信息学分析表明Bxy-octr-1在进化上是保守的。实时定量PCR数据显示，Bxy-octr-1的表达需要在整个生命的B。木生植物mRNA原位杂交结果表明，Bxy-octr-1主要分布在B的头咽部、体壁肌肉、肠道和性腺器官中。木生植物RNA干扰（RNAi）结果显示，胚胎的孵化率和运动速度均显著降低。RNAi处理后的第二阶段幼鱼出现了明显的异常表型。此外，它的个体发育也受到阻碍。Bxy-octr-1基因的缺失降低了雌虫的生殖力，其中31.25%的雌虫不能排卵。此外，线虫的错误定位率显著增加。我们的研究表明Bxy-octr-1在B的运动、早期个体发育和交配行为中是必不可少的。木生植物
Bursaphelenchus xylophilus is an invasive plant-parasitic nematode causing the notorious pine wilt disease (PWD) worldwide, which results in huge economic losses. G protein-coupled receptors (GPCRs) play an essential role in mating and reproduction behavior of animals. As a unique biogenic amine in invertebrates, octopamine (OA) can regulate a variety of physiological and behavioral responses by binding specific GPCRs. These specific GPCRs are also called octopamine receptors (OARs), and octr-1 is one of them. However, Bxy-octr-1 is unknown in B. xylophilus. Therefore, we investigated the expression pattern and biological function of Bxy-octr-1. Bioinformatics analysis indicated that Bxy-octr-1 was evolutionarily conserved. The real-time quantitative PCR data revealed that Bxy-octr-1 expression was required throughout the entire life of B. xylophilus. mRNA in situ hybridization showed that Bxy-octr-1 was mainly located in the cephalopharynx, body wall muscle, intestine, and gonadal organs of B. xylophilus. RNA interference (RNAi) showed that embryo hatching rates and locomotion speeds were both dramatically decreased. Obvious abnormal phenotypes were observed in the second-stage of juveniles after RNAi treated. Furthermore, its ontogenesis was stunting. Lack of Bxy-octr-1 reduced fecundity of females, of which 31.25% of them could not successfully ovulate. In addition, the error positioning ratio of the nematode was significantly increased. Our study suggests that Bxy-octr-1 is indispensable for locomotion, early ontogenesis and mating behavior in B. xylophilus.