Mass Spectrometry in High Throughput Screening: A Case Study on Acetyl-Coenzyme A Carboxylase using RapidFire® - Mass Spectrometry (RF-MS)
Mass Spectrometry in High Throughput Screening: A Case Study on Acetyl-Coenzyme A Carboxylase using RapidFire® - Mass Spectrometry (RF-MS)
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DOI：
10.2174/138620709789104924
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发表时间：
2009-09-01
影响因子：
1.8
通讯作者：
Ozbal, Can
中科院分区：
文献类型：
--
作者：
Jonas, Maxine;LaMarr, William A.;Ozbal, Can
在这篇综述中，讨论了在高通量分析中使用质谱的各种技术和方法。重点介绍了基于四极杆的质谱法在针对酶靶标筛选化学库以鉴定抑制剂和/或激活剂中的应用。详细描述了 RapidFire 质谱系统，这是一种与三重四极杆质谱仪连接的集成在线固相萃取系统，并描述了乙酰辅酶 A 羧化酶 (ACC) 测定的一系列抑制剂的鉴定。
In this review various technologies and approaches for the utilization of mass spectrometry in high throughput analyses are discussed. The use of quadrupole-based mass spectrometry in the screening of chemical libraries against enzymatic targets for the identification of inhibitors and/or activators is highlighted. The RapidFire mass spectrometry system, an integrated on-line solid-phase extraction system interfaced to a triple-quadrupole mass spectrometer is described in detail, and the identification of a series of inhibitors of the acetyl-coenzyme A carboxylase (ACC) assay is described.