Quantifying the concentration and penetration depth of long-lived RONS in plasma-activated water by UV absorption spectroscopy
Quantifying the concentration and penetration depth of long-lived RONS in plasma-activated water by UV absorption spectroscopy
复制标题
通过紫外吸收光谱定量等离子活化水中长寿命 RONS 的浓度和渗透深度
DOI：
10.1063/1.5037660
复制
发表时间：
2019-01-01
期刊：
影响因子：
1.6
通讯作者：
Kong, Michael G.
中科院分区：
文献类型：
--
作者：
Liu, Zhijie;Zhou, Chunxi;Kong, Michael G.
活性氧和活性氮物质（RONS）被认为在生物医学应用中起着关键作用，这意味着RONS必须到达靶组织才能产生治疗效果。确定RONS浓度的现有方法（电子自旋光谱法和微孔板阅读）不适合于实验实时测量，因为它们需要向等离子体处理的介质中添加指示试剂，这可能改变介质的化学组成。本文提出了一种利用紫外吸收光谱测量等离子体活化水（PAW）中长寿命RONS浓度的方法。基于标准溶液（H2 O2，NaNO 2和NaNO 3）的吸收光谱的分析和拟合，我们提出了一个详细的拟合程序，使我们能够计算单纯H2 O2，NO2-和NO3-的浓度。结果表明，PAW溶液的pH值和RONS之间的交叉反应性与吸收光谱有很强的相关性。为了确认计算的准确性，我们还使用酶标仪并添加化学试剂来测量H2 O2，NO2−和NO3−的浓度。结果表明，用本文提出的拟合方法计算的浓度是比较准确的，误差范围是可以接受的。此外，RONS在PAW中的时间依赖性扩散的测量和分析在不同深度的PAW。这种拟合方法构成了一种非侵入性的方法来测量RONS在不同深度的PAW。活性氧和活性氮物种（RONS）被认为是在生物医学应用中发挥关键作用，这意味着RONS必须达到靶组织产生治疗效果。确定RONS浓度的现有方法（电子自旋光谱法和微孔板阅读）不适合于实验实时测量，因为它们需要向等离子体处理的介质中添加指示试剂，这可能改变介质的化学组成。本文提出了一种利用紫外吸收光谱测量等离子体活化水（PAW）中长寿命RONS浓度的方法。基于标准溶液（H2 O2，NaNO 2和NaNO 3）的吸收光谱的分析和拟合，我们提出了一个详细的拟合程序，使我们能够计算单纯H2 O2，NO2-和NO3-的浓度。结果表明，PAW溶液的pH值和RONS之间的交叉反应性与吸收光谱有很强的相关性。为了确认...
Reactive oxygen and reactive nitrogen species (RONS) are believed to play a key role in biomedical applications, which means that RONS must reach the target tissue to produce a therapeutic effect. Existing methods (electron spin spectrometry and microplate reading) to determine the RONS concentration are not suitable for experimental real-time measurements because they require adding an indicating reagent to the plasma-treated medium, which may alter the chemical composition of the medium. In this paper, we propose a method to measure the long-lived RONS concentration in plasma-activated water (PAW) by using UV absorption spectroscopy. Based on an analysis and fit of the absorption spectra of standard solutions (H2O2, NaNO2, and NaNO3), we propose a detailed fitting procedure that allows us to calculate the concentrations of simplex H2O2, NO2−, and NO3−. The results show that the pH and the cross reactivity between RONS in PAW correlate strongly with the absorption spectra. To confirm the accuracy of the calculations, we also use a microplate reader and add chemical reagents to measure the concentrations of H2O2, NO2−, and NO3−. The results show that the concentrations calculated by the proposed fitting method are relatively accurate and that the error range is acceptable. Additionally, the time-dependent diffusion of RONS in PAW is measured and analyzed at different depths in the PAW. This fitting approach constitutes a nonintrusive approach to measure RONS at different depths in PAW.Reactive oxygen and reactive nitrogen species (RONS) are believed to play a key role in biomedical applications, which means that RONS must reach the target tissue to produce a therapeutic effect. Existing methods (electron spin spectrometry and microplate reading) to determine the RONS concentration are not suitable for experimental real-time measurements because they require adding an indicating reagent to the plasma-treated medium, which may alter the chemical composition of the medium. In this paper, we propose a method to measure the long-lived RONS concentration in plasma-activated water (PAW) by using UV absorption spectroscopy. Based on an analysis and fit of the absorption spectra of standard solutions (H2O2, NaNO2, and NaNO3), we propose a detailed fitting procedure that allows us to calculate the concentrations of simplex H2O2, NO2−, and NO3−. The results show that the pH and the cross reactivity between RONS in PAW correlate strongly with the absorption spectra. To confirm the accuracy of the ...