Evidence for novel and specialized mycorrhizal parasitism: the orchid Gastrodia confusa gains carbon from saprotrophic Mycena
Evidence for novel and specialized mycorrhizal parasitism: the orchid Gastrodia confusa gains carbon from saprotrophic Mycena
复制标题
DOI：
10.1098/rspb.2008.1225
复制
发表时间：
2009-02-22
影响因子：
4.7
通讯作者：
Yukawa, Tomohisa
中科院分区：
文献类型：
--
作者：
Ogura-Tsujita, Yuki;Gebauer, Gerhard;Yukawa, Tomohisa
对亚洲非光合型兰花天麻的生理生态进行了研究。我们通过分子鉴定揭示了它的菌根伙伴，并通过分析碳和氮的天然稳定同位素丰度确定了它的最终营养来源。利用内部转录间隔区和大亚基nrDNA序列进行的分子鉴定表明，海藻与几种凋落物和木材分解真菌Mycena存在联系。利用光合作用植物标准样品和外生菌根上寄生单胞菌的样品，分析了拟棉的碳、氮同位素特征。我们发现，海藻在13C中高度富含，但在N-15中含量不高，而Unflora在C-13和N-15中都高度富含。海藻的C-13和N-15特征与腐生菌的子实体特征最接近。我们的结果首次使用分子和质谱学方法证明，真菌异养植物通过寄生木材或枯枝腐烂的真菌获得碳。此外，我们还证明，一些原本自由生活的非菌根真菌Mycena可以成为兰花的菌根伙伴。
We investigated the physiological ecology of the Asian non-photosynthetic orchid Gastrodia confusa. We revealed its mycorrhizal partners by using molecular identification and identified its ultimate nutritional source by analysing carbon and nitrogen natural stable isotope abundances. Molecular identification using internal transcribed spacer and large subunit nrDNA sequences showed that G. confusa associates with several species of litter- and wood-decomposer Mycena fungi. The carbon and nitrogen isotope signatures of G. confusa were analysed together with photosynthetic plant reference samples and samples of the ectomycorrhizal epiparasite Monotropa uniflora. We found that G. confusa was highly enriched in 13 C but not greatly in N-15, while M. uniflora was highly enriched in both C-13 and N-15. The C-13 and N-15 signatures of G. confusa were the closest to those of the fruit bodies of saprotrophic fungi. Our results demonstrate for the first time using molecular and mass-spectrometric approaches that myco-heterotrophic plants gain carbon through parasitism of wood or litter decaying fungi. Furthermore, we demonstrate that, several otherwise free-living non-mycorrhizal, Mycena can be mycorrhizal partners of orchids.